DETERMINATION OF BIOAVAILABLE PHOSPHORUS IN FISH FEEDS BY ULTRASOUND EXTRACTION AND VISIBLE SPECTROPHOTOMETRY

被引：13

作者：

de Moraes, Paula M. ^{[1
]}

Loureiro, Vanessa R. ^{[1
]}

Padilha, Pedro M. ^{[1
]}

Neves, Renato de Cassio F. ^{[2
]}

Saleh, Mayra A. D. ^{[2
]}

dos Santos, Felipe A. ^{[2
]}

Silva, Fabio A. ^{[2
]}

机构：

[1] Univ Estadual Paulista, Inst Biociencias, Dept Quim & Bioquim, BR-18618000 Botucatu, SP, Brazil

[2] Univ Estadual Paulista, Fac Med Vet & Zootecn, BR-18618000 Botucatu, SP, Brazil

来源：

QUIMICA NOVA | 2009年 / 32卷 / 04期

关键词：

ultrasound extraction; fish nutrition; phosphorus absorption; ASSISTED EXTRACTION; APPARENT DIGESTIBILITY; SLURRIES; PHYTASE; SUPPLEMENTATION; DIGESTION; SAMPLES; GFAAS; FECES; ZINC;

D O I：

10.1590/S0100-40422009000400018

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The aim of the present work was to develop and optimize a method for determination of bioavailable phosphorus in samples of feces and fish feed using ultrasound extraction and subsequent quantification by visible spectrophotometry. Using as extractor solution HNO3 0.50 mol L-1, the great conditions of extraction established were: sample mass - 100 mg, samples granulometry - < 60 mu m, sonification time - five cycles of 40 s and ultrasound potency - 136 W. The proposed method was applied in studies of digestibility of this nutrient in different feeds used in diets of juvenile of Nile tilapia.

引用

页码：923 / 927

页数：5

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