Interdomain Flexibility in Full-length Matrix Metalloproteinase-1 (MMP-1)

被引：60

作者：

Bertini, Ivano ^{[1
,2
]}

Fragai, Marco ^{[1
,3
]}

Luchinat, Claudio ^{[1
,3
]}

Melikian, Maxime ^{[1
]}

Mylonas, Efstratios ^{[4
]}

Sarti, Niko ^{[1
,2
]}

Svergun, Dmitri I. ^{[4
,5
]}

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

[3] Univ Florence, Dept Agr Biotechnol, I-50144 Florence, Italy

[4] Hamburg Outstn, European Mol Biol Lab, D-22603 Hamburg, Germany

[5] Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 19期

关键词：

C-TERMINAL DOMAIN; NEUTROPHIL COLLAGENASE; CRYSTAL-STRUCTURE; CATALYTIC DOMAIN; SUBSTRATE-SPECIFICITY; SURFACE ACCESSIBILITY; ACTIVATION MECHANISM; TISSUE INHIBITORS; PEPTIDE MODELS; NMR RELAXATION;

D O I：

10.1074/jbc.M809627200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of extensive reciprocal conformational freedom between the catalytic and the hemopexin-like domains of fulllength matrix metalloproteinase-1 (MMP-1) is demonstrated by NMR and small angle x-ray scattering experiments. This finding is discussed in relation to the essentiality of the hemopexin-like domain for the collagenolytic activity of MMP-1. The conformational freedom experienced by the present system, having the shortest linker between the two domains, when compared with similar findings on MMP-12 and MMP-9 having longer and the longest linker within the family, respectively, suggests this type of conformational freedom to be a general property of all MMPs.

引用

收藏

页码：12821 / 12828

页数：8

相关论文

共 66 条

[1] A paramagnetic probe to localize residues next to carboxylates on protein surfaces [J].

Aime, S ;

D'Amelio, N ;

Fragai, M ;

Lee, YM ;

Luchinat, C ;

Terreno, E ;

Valensin, G .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2002, 7 (06) :617-622

[2] Bioinformatic comparison of structures and homology-models of matrix metalloproteinases [J].

Andreini, C ;

Banci, L ;

Bertini, I ;

Luchinat, C ;

Rosato, A .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (01) :21-31

[3] TIMP-1 contact sites and perturbations of stromelysin 1 mapped by NMR and a paramagnetic surface probe [J].

Arumugam, S ;

Hemme, CL ;

Yoshida, N ;

Suzuki, K ;

Nagase, H ;

Bejanskii, M ;

Wu, B ;

Van Doren, SR .

BIOCHEMISTRY, 1998, 37 (27) :9650-9657

[4] Structural characterization of flexible proteins using small-angle X-ray scattering [J].

Bernado, Pau ;

Mylonas, Efstratios ;

Petoukhov, Maxim V. ;

Blackledge, Martin ;

Svergun, Dmitri I. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (17) :5656-5664

[5] NMR studies on the surface accessibility of the archaeal protein Sso7d by using TEMPOL and Gd(III)(DTPA-BMA) as paramagnetic probes [J].

Bernini, Andrea ;

Venditti, Vincenzo ;

Spiga, Ottavia ;

Ciutti, Arianna ;

Prischi, Filippo ;

Consonni, Roberto ;

Zetta, Lucia ;

Arosio, Ivana ;

Fusi, Paola ;

Guagliardi, Annamaria ;

Niccolai, Neri .

BIOPHYSICAL CHEMISTRY, 2008, 137 (2-3) :71-75

[6] Paramagnetic metal ions in ligand screening:: The CoII matrix metalloproteinase 12 [J].

Bertini, I ;

Fragai, M ;

Lee, YM ;

Luchinat, C ;

Terni, B .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (17) :2254-2256

[7] X-ray structures of binary and ternary enzyme-product-Inhibitor complexes of matrix metalloproteinases [J].

Bertini, I ;

Calderone, V ;

Fragai, M ;

Luchinat, C ;

Mangani, S ;

Terni, B .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (23) :2673-2676

[8] Conformational variability of matrix metalloproteinases: Beyond a single 3D structure [J].

Bertini, I ;

Calderone, V ;

Cosenza, M ;

Fragai, M ;

Lee, YM ;

Luchinat, C ;

Mangani, S ;

Terni, B ;

Turano, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (15) :5334-5339

[9] Evidence of reciprocal reorientation of the catalytic and hemopexin-like domains of full-length MMP-12 [J].

Bertini, Ivano ;

Calderone, Vito ;

Fragai, Marco ;

Jaiswal, Rahul ;

Luchinat, Claudio ;

Melikian, Maxime ;

Mylonas, Efstratios ;

Svergun, Dmitri I. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (22) :7011-7021

[10] Snapshots of the reaction mechanism of matrix metalloproteinases [J].

Bertini, Ivano ;

Calderone, Vito ;

Fragai, Marco ;

Luchinat, Claudio ;

Maletta, Massimiliano ;

Yeo, Kwon Joo .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (47) :7952-7955

← 1 2 3 4 5 6 7 →