Mandelate racemase activity in ionic liquids: scopes and limitations

被引：22

作者：

Kaftzik, N

Kroutil, W

Faber, K

Kragl, U

机构：

[1] Univ Rostock, Dept Chem, D-18059 Rostock, Germany

[2] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz, Austria

来源：

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL | 2004年 / 214卷 / 01期

关键词：

ionic liquids; dynamic resolution; mandelate racemase; water activity;

D O I：

10.1016/j.molcata.2003.11.034

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Ionic liquids (IL) offer new possibilities for solvent engineering for biocatalytic reactions. The deracemization of (+/-)-mandelic acid using a lipase-mandelate racemase two-enzyme system was used to investigate the scopes and limitations of ionic liquids as new reaction media for a dynamic resolution approach. Mandelate racemase [EC 5.1.2.2] from Pseudomonas putida ATCC 12633 was observed to be active in ionic liquids such as 1,3-dimethylimidazolium methylsulfate ([MMIM][MeSO4]) or 1-butyl-3-methylimidazolium octylsulfate, ([BMIM][OctSO(4)]) at water activities a(w) > 0.74. Mandelate racemase activity could also be obtained in a biphasic system consisting of water and 1-octyl-3-methylimidazolium hexafluorophosphate ([OMIM][PF6]) in a ratio of 1:10. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：107 / 112

页数：6

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