Targeted Retrieval and Analysis of Five Neandertal mtDNA Genomes

被引：343

作者：

Briggs, Adrian W. ^{[1
]}

Good, Jeffrey M. ^{[1
]}

Green, Richard E. ^{[1
]}

Krause, Johannes ^{[1
]}

Maricic, Tomislav ^{[1
]}

Stenzel, Udo ^{[1
]}

Lalueza-Fox, Carles ^{[2
]}

Rudan, Pavao

Brajkovic, Dejana ^{[3
]}

Kucan, Zeljko

Gusic, Ivan

Schmitz, Ralf ^{[4
,5
]}

Doronichev, Vladimir B. ^{[6
]}

Golovanova, Liubov V. ^{[6
]}

de la Rasilla, Marco ^{[7
]}

Fortea, Javier ^{[7
]}

Rosas, Antonio ^{[8
]}

Paeaebo, Svante ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany

[2] Univ Pompeu Fabra, CSIC, Inst Evolutionary Biol, Barcelona 08003, Spain

[3] Croatian Acad Sci & Arts, Inst Quaternay Paleontol & Geol, HR-10000 Zagreb, Croatia

[4] Landschaftsverband Rheinland Landesmuseum, D-53115 Bonn, Germany

[5] Univ Tubingen, Dept Early Prehist & Quaternary Ecol, D-72074 Tubingen, Germany

[6] Lab Prehist, St Petersburg, Russia

[7] Univ Oviedo, Dept Hist, Area Prehist, Oviedo, Spain

[8] CSIC, Museo Nacl Ciencias Nat, Dept Paleobiol, E-28006 Madrid, Spain

来源：

SCIENCE | 2009年 / 325卷 / 5938期

关键词：

ANCIENT DNA; MITOCHONDRIAL-DNA; REVEALS; POPULATIONS; SEQUENCES; SELECTION; GENETICS; REMAINS; SIMCOAL; HUMANS;

D O I：

10.1126/science.1174462

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Analysis of Neandertal DNA holds great potential for investigating the population history of this group of hominins, but progress has been limited due to the rarity of samples and damaged state of the DNA. We present a method of targeted ancient DNA sequence retrieval that greatly reduces sample destruction and sequencing demands and use this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. We find that mtDNA genetic diversity in Neandertals that lived 38,000 to 70,000 years ago was approximately one-third of that in contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.

引用

页码：318 / 321

页数：4

共 30 条

[1] Serial SimCoal: A population genetics model for data from multiple populations and points in time [J].

Anderson, CNK ;

Ramakrishnan, U ;

Chan, YL ;

Hadly, EA .

BIOINFORMATICS, 2005, 21 (08) :1733-1734

[2] Fishing for ancient DNA [J].

Anderung, Cecilia ;

Persson, Per ;

Bouwman, Abigail ;

Elburg, Rengert ;

Goethertroem, Anders .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2008, 2 (02) :104-107

[3] Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA [J].

Andrews, RM ;

Kubacka, I ;

Chinnery, PF ;

Lightowlers, RN ;

Turnbull, DM ;

Howell, N .

NATURE GENETICS, 1999, 23 (02) :147-147

[4] MtDNA variation predicts population size in humans and reveals a major southern Asian chapter in human prehistory [J].

Atkinson, Quentin D. ;

Gray, Russell D. ;

Drummond, Alexei J. .

MOLECULAR BIOLOGY AND EVOLUTION, 2008, 25 (02) :468-474

[5] Identification of ancient remains through genomic sequencing [J].

Blow, Matthew J. ;

Zhang, Tao ;

Woyke, Tanja ;

Speller, Camilla F. ;

Krivoshapkin, Andrei ;

Yang, Dongya Y. ;

Derevianko, Anatoly ;

Rubin, Edward M. .

GENOME RESEARCH, 2008, 18 (08) :1347-1353

[6] Patterns of damage in genomic DNA sequences from a Neandertal [J].

Briggs, Adrian W. ;

Stenzel, Udo ;

Johnson, Philip L. F. ;

Green, Richard E. ;

Kelso, Janet ;

Pruefer, Kay ;

Meyer, Matthias ;

Krause, Johannes ;

Ronan, Michael T. ;

Lachmann, Michael ;

Paeaebo, Svante .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (37) :14616-14621

[7] Novel high-resolution characterization of ancient DNA reveals C>U-type base modification events as the sole cause of post mortem miscoding lesions [J].

Brotherton, Paul ;

Endicott, Phillip ;

Sanchez, Juan J. ;

Beaumont, Mark ;

Barnett, Ross ;

Austin, Jeremy ;

Cooper, Alan .

NUCLEIC ACIDS RESEARCH, 2007, 35 (17) :5717-5728

[8] BEAST: Bayesian evolutionary analysis by sampling trees [J].

Drummond, Alexei J. ;

Rambaut, Andrew .

BMC EVOLUTIONARY BIOLOGY, 2007, 7 (1)

[9] SIMCOAL: A general coalescent program for the simulation of molecular data in interconnected populations with arbitrary demography [J].

Excoffier, L ;

Novembre, J ;

Schneider, S .

JOURNAL OF HEREDITY, 2000, 91 (06) :506-509

[10] Neandertal genetic diversity: A fresh look from old samples [J].

Excoffier, Laurent .

CURRENT BIOLOGY, 2006, 16 (16) :R650-R652

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