Electrolyzed-reduced water reduced hemodialysisinduced erythrocyte impairment in end-stage renal disease patients

被引：45

作者：

Huang, K-C

Yang, C-C

Hsu, S-P

Lee, K-T

Liu, H-W

Morisawa, S.

Otsubo, K.

Chien, C-T

机构：

[1] Natl Taiwan Univ Hosp, Dept Med Res, Taipei, Taiwan

[2] Natl Taiwan Univ, Coll Med, Dept Family Med, Taipei 10764, Taiwan

[3] Taipei City United Heping Hosp, Taipei, Taiwan

[4] Natl Yang Ming Univ, Dept Internal Med, Taipei 112, Taiwan

[5] Natl Taiwan Univ, Coll Med, Dept Med Res, Taipei 10764, Taiwan

[6] Wan Hwa Reg Hosp, Taipei, Taiwan

[7] Natl Taiwan Univ Sci & Technol, Dept Polymer Engn, Taipei, Taiwan

[8] Nihon Trim Co Ltd, Osaka, Japan

来源：

KIDNEY INTERNATIONAL | 2006年 / 70卷 / 02期

关键词：

hemodialysis; hemolysis; oxidative stress; electrolyzed-reduced water;

D O I：

10.1038/sj.ki.5001576

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Chronic hemodialysis (HD) patients increase erythrocyte susceptibility to hemolysis and impair cell survival. We explored whether electrolyte-reduced water (ERW) could palliate HD-evoked erythrocyte impairment and anemia. Forty-three patients undergoing chronic HD were enrolled and received ERW administration for 6 month. We evaluated oxidative stress in blood and plasma, erythrocyte methemoglobin (metHb)/ferricyanide reductase activity, plasma metHb, and proinflammatory cytokines in the chronic HD patients without treatment (n = 15) or with vitamin C (VC)- (n = 15), vitamin E (VE)-coated dialyzer (n = 15), or ERW treatment (n = 15) during an HD course. The patients showed marked increases (15-fold) in blood reactive oxygen species, mostly H2O2, after HD without any treatment. HD resulted in decreased plasma VC, total antioxidant status, and erythrocyte metHb/ferricyanide reductase activity and increased erythrocyte levels of phosphatidylcholine hydroperoxide ( PCOOH) and plasma metHb. Antioxidants treatment significantly palliated single HD course-induced oxidative stress, plasma and RBC PCOOH, and plasma metHb levels, and preserved erythrocyte metHb/ferricyanide reductase activity in an order VC > ERW > VE-coated dialyzer. However, ERW had no side effects of oxalate accumulation easily induced by VC. Six-month ERW treatment increased hematocrit and attenuated proinflammatory cytokines profile in the HD patients. In conclusion, ERW treatment administration is effective in palliating HD-evoked oxidative stress, as indicated by lipid peroxidation, hemolysis, and overexpression of proinflammatory cytokines in HD patients.

引用

页码：391 / 398

页数：8

共 49 条

[11] Seven new mutations in the nicotinamide adenine dinucleotide reduced-cytochrome b5 reductase gene leading to methemoglobinemia type I [J].