Fabrication of 2D-protein arrays using biotinylated thiols:: results from fluorescence microscopy and atomic force microscopy

被引：19

作者：

Grunwald, C ^{[1
]}

Eck, W

Opitz, N

Kuhlmann, J

Wölf, C

机构：

[1] Ruhr Univ Bochum, D-44780 Bochum, Germany

[2] Univ Heidelberg, D-69120 Heidelberg, Germany

[3] Max Planck Inst Mol Physiol, D-44202 Dortmund, Germany

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2004年 / 6卷 / 17期

关键词：

D O I：

10.1039/b405543h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The coupling of a fluorescently labelled protein, streptavidin, to biotinylated organic surfaces is investigated using fluorescence microscopy (FM) and atomic force microscopy (AFM). In order to study the importance of nonspecific adsorption investigations were carried out using two-dimensionally structured self-assembled monolayers (SAMs), which were fabricated using micro-contact printing. The relative amount of specific vs. non-specific adsorption could be readily determined by comparing the amount of streptavidin adsorbed on adjacent regions consisting of biotinylated organothiolates and of protein-resistant oligoethyleneglycol (OEG)-thiols.

引用

页码：4358 / 4362

页数：5

共 19 条

[1]

BARNARD G, 1986, METHOD ENZYMOL, V133, P284

[2]

BOOTH LR, 2002, AM BIOTECHNOL LAB, P76

[3] Long-term stability of self-assembled monolayers in biological media [J].