HIV-1 protease inhibition and anti-HIV effect of natural and synthetic water-soluble lignin-like substances

被引：42

作者：

Ichimura, T

Otake, T

Mori, H

Maruyama, S

机构：

[1] Natl Inst Biosci & Human Technol, Agcy Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan

[2] Osaka Prefectural Inst Publ Hlth, Higashinari Ku, Osaka 5370025, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 12期

关键词：

HIV-1; protease; lignin; MT-4; cells;

D O I：

10.1271/bbb.63.2202

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Water-soluble lignin extracted from natural sources and dehydrogenated polymers of p-coumaric acid and ferulic acid inhibited HIV-l protease activity. The dehydrogenated polymers, which are thought to be model compounds for lignin, were synthesized and fractionated into four ranges of molecular mass by ultra-filtration: Le., over 30 kDa, 30-10 kDa, 10-1 kDa and 1 kDa-500 Da. All of these fractions had HIV-1 protease inhibitory activity. The anti-HIV-l effect of the smallest mass fractions of the dehydrogenated polymers (I kDa-500 Da) was also tested, and it was found that these fractions inhibited the replication of HIV-1 in MT-4 cells.

引用

页码：2202 / 2204

页数：3

共 11 条

[1] SPECTROPHOTOMETRIC ASSAY AND PROPERTIES OF ANGIOTENSIN-CONVERTING ENZYME OF RABBIT LUNG [J].