Ratiometric porphyrin-based layers and nanoparticles for measuring oxygen in biosamples

被引：52

作者：

Cywinski, Piotr J. ^{[1
]}

Moro, Artur J. ^{[1
]}

Stanca, Sarmiza E. ^{[2
]}

Biskup, Christoph ^{[2
]}

Mohr, Gerhard J. ^{[1
]}

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Univ Klinikum Jena, Inst Physiol 2, D-07743 Jena, Germany

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 135卷 / 02期

关键词：

Porphyrins; Microemulsion polymerization; Nanosensor; Oxygen measurement; Luminescence spectroscopy; CHEMICAL SENSORS; COPOLYMERS; BEADS;

D O I：

10.1016/j.snb.2008.09.039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Oxygen-sensitive polystyrene layers and nanoparticles have been developed for measuring oxygen in biosamples. Polymer layers were prepared via spin-coating on glass plates while the nanoparticles were synthesized by microemulsion polymerization. Platinum(II)meso-tetra(pentafluorophenyl)porphine (PtTFPP) was used as an oxygen sensor whereas N,N'-bis(1-hexylheptyl)perylene-3,4:9,10-bis(dicarboximide) (S13) was used as a reference dye. For both, layers and nanoparticles, the sensor response was assessed in aqueous solution as well as in yeast culture. Quenching of porphyrin phosphorescence by oxygen was observed whereas fluorescence of the reference dye remained essentially unaffected. By using the signal of the reference dye fluctuations in the intensity of the excitation light or the nanosensor concentration can be easily corrected. Both sensor systems were compared with respect to their spectral response and their sensitivity. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：472 / 477

页数：6

共 28 条

[1] Platinum tetrakis(pentafluorophenyl)porphyrin immobilized in polytrifluoroethylmethacrylate film as a photostable optical oxygen detection material [J].