A Shape- Adaptive, Antibacterial- Coating of Immobilized Quaternary- Ammonium Compounds Tethered on Hyperbranched Polyurea and its Mechanism of Action

被引：369

作者：

Asri, Lia A. T. W. ^{[1
]}

Crismaru, Mihaela ^{[2
,3
]}

Roest, Steven ^{[2
,3
]}

Chen, Yun ^{[2
,3
]}

Ivashenko, Oleksii ^{[4
]}

Rudolf, Petra ^{[4
]}

Tiller, Joerg C. ^{[5
]}

van der Mei, Henny C. ^{[2
,3
]}

Loontjens, Ton J. A. ^{[1
,2
,3
]}

Busscher, Henk J. ^{[2
,3
]}

机构：

[1] Univ Groningen, Zernike Inst Adv Mat, Dept Polymer Chem, NL-9747 AG Groningen, Netherlands

[2] Univ Groningen, NL-9713 AV Groningen, Netherlands

[3] Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn, NL-9713 AV Groningen, Netherlands

[4] Univ Groningen, Zernike Inst Adv Mat, Surfaces & Thin Films Grp, NL-9747 AG Groningen, Netherlands

[5] TU Dortmund, Dept Bio & Chem Engn, D-44227 Dortmund, Germany

来源：

ADVANCED FUNCTIONAL MATERIALS | 2014年 / 24卷 / 03期

关键词：

antibacterials; hyperbranched; polyurea; polyethyleneimine; quaternary ammonium; atomic force microscopy; SELF-ASSEMBLED MONOLAYERS; FORCE MICROSCOPY; ALKYLATED POLYETHYLENIMINE; ANTIMICROBIAL COATINGS; CATIONIC SURFACES; ADHESION FORCES; IN-VIVO; BACTERIA; NANOPARTICLES; PROTEINS;

D O I：

10.1002/adfm.201301686

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Quaternary-ammonium-compounds are potent cationic antimicrobials used in everyday consumer products. Surface-immobilized, quaternary-ammonium-compounds create an antimicrobial contact-killing coating. We describe the preparation of a shape-adaptive, contact-killing coating by tethering quaternary-ammonium-compounds onto hyperbranched polyurea coatings, able to kill adhering bacteria by partially enveloping them. Even after extensive washing, coatings caused high contact-killing of Staphylococcus epidermidis, both in culture-based assays and through confocal-laser-scanning-microscopic examination of the membrane-damage of adhering bacteria. In culture-based assays, at a challenge of 1600 CFU/cm(2), contact-killing was >99.99%. The working-mechanism of dissolved quaternary-ammonium-compounds is based on their interdigitation in bacterial membranes, but it is difficult to envisage how immobilized quaternary-ammonium-molecules can exert such a mechanism of action. Staphylococcal adhesion forces to hyperbranched quaternary-ammonium coatings were extremely high, indicating that quaternary-ammonium-molecules on hyperbranched polyurea partially envelope adhering bacteria upon contact. These lethally strong adhesion forces upon adhering bacteria then cause removal of membrane lipids and eventually lead to bacterial death.

引用

页码：346 / 355

页数：10

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