Determination of pKa values of quinolones from mobility and spectroscopic data obtained by capillary electrophoresis and a diode array detector

被引：140

作者：

Jiménez-Lozano, E

Marqués, I

Barrón, D

Beltrán, JL

Barbosa, J

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

[2] Univ Girona, Fac Sci, Dept Chem, Girona 17071, Spain

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 464卷 / 01期

关键词：

capillary electrophoresis; diode array detector; dissociation constant;

D O I：

10.1016/S0003-2670(02)00435-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work compares the values of dissociation constants obtained from electrophoretic mobilities of a series of quinolones at different pH values and those obtained using absorbance spectra at the maximum of the eletrophoretic peaks. The results obtained show that the two methods are complementary and constitute a valuable means of obtaining better precision. The two methods proposed can be used simultaneously without an increase in the experimental time and allow confirmation of the results obtained. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：37 / 45

页数：9

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共 35 条

[1]

[Anonymous], [No title captured]

[2] Factor analysis applied to correlation between acidity constants of series of diuretics, quinolones and buffers, with solvatochromic parameters in water-acetonitrile mixtures [J].

Barbosa, J ;

Marques, I ;

Fonrodona, G ;

Barron, D ;

Berges, R .

ANALYTICA CHIMICA ACTA, 1997, 347 (03) :385-393

[3] Protonation equilibria of quinolone antibacterials in acetonitrile-water mobile phases used in LC [J].

Barbosa, J ;

Berges, R ;

Toro, I ;

SanzNebot, V .

TALANTA, 1997, 44 (07) :1271-1283

[4] Retention behaviour of quinolone derivatives in high-performance liquid chromatography -: Effect of pH and evaluation of ionization constants [J].

Barbosa, J ;

Bergés, R ;

Nebot, VS .

JOURNAL OF CHROMATOGRAPHY A, 1998, 823 (1-2) :411-422

[5] Secondary chemical equilibria in high-performance liquid chromatography: influence of ionic strength and pH on retention of peptides [J].

Barbosa, J ;

Toro, I ;

Sanz-Nebot, V .

JOURNAL OF CHROMATOGRAPHY A, 1998, 823 (1-2) :497-509

[6] Electrophoretic behaviour of quinolones in capillary electrophoresis -: Effect of pH and evaluation of ionization constants [J].

Barbosa, J ;

Barrón, D ;

Jiménez-Lozano, E .

JOURNAL OF CHROMATOGRAPHY A, 1999, 839 (1-2) :183-192

[7] Retention behaviour of peptides, quinolones, diuretics and peptide hormones in liquid chromatography -: Influence of ionic strength and pH on chromatographic retention [J].

Barbosa, J ;

Toro, I ;

Bergés, R ;

Sanz-Nebot, V .

JOURNAL OF CHROMATOGRAPHY A, 2001, 915 (1-2) :85-96

[8] Comparison between capillary electrophoresis, liquid chromatography, potentiometric and spectrophotometric techniques for evaluation of pKa values of zwitterionic drugs in acetonitrile-water mixtures [J].

Barbosa, J ;

Barrón, D ;

Jiménez-Lozano, E ;

Sanz-Nebot, V .

ANALYTICA CHIMICA ACTA, 2001, 437 (02) :309-321

[9] The application of factor analysis to solvatochromic parameters and pHs values for the standardization of potentiometric sensors in mobile phases used in liquid chromatography [J].

Barbosa, J ;

Marqués, I ;

Barrón, D ;

Sanz-Nebot, V .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1999, 18 (08) :543-549

[10] Prediction of electrophoretic behaviour of a series of quinolones in aqueous methanol [J].

Barrón, D ;

Jimenez-Lozano, E ;

Barbosa, J .

JOURNAL OF CHROMATOGRAPHY A, 2001, 919 (02) :395-406

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