Prediction of electrophoretic behaviour of a series of quinolones in aqueous methanol

被引：29

作者：

Barrón, D ^{[1
]}

Jimenez-Lozano, E ^{[1
]}

Barbosa, J ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 919卷 / 02期

关键词：

buffer composition; dissociation constants; electrophoretic mobility; pH effects; resolution prediction; quinolones;

D O I：

10.1016/S0021-9673(01)00814-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quinolones are a family of antibacterial agents used in human and veterinary clinics. The examination of protonation equilibria is essential because their antibacterial activity is pH-dependent. In this work, dissociation constants of quinolones in MeOH-water mixtures were obtained using capillary electrophoresis. The method is based on a model that relates electrophoretic mobility of the solute with pH. The effect of pH, pK(a) and activity coefficient on electrophoretic behaviour was considered. Standard pH values for buffer solutions were previously determined in MeOH-water mixtures, and the pH can thus be measured in these media as in water. This model is also used to obtain the optimum conditions for the separation of a series of substances because it allows one to predict the resolution between adjacent peaks from a few experimental data. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：395 / 406

页数：12

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