Exosomal miR-486 regulates hypoxia-induced erythroid differentiation of erythroleukemia cells through targeting Sirt1

被引：48

作者：

Shi, Xue-Feng ^{[1
,2
,4
]}

Wang, Hua ^{[3
,5
]}

Kong, Fan-Xuan ^{[3
,5
]}

Xu, Qin-Qin ^{[1
,2
,4
]}

Xiao, Feng-Jun ^{[3
,5
]}

Yang, Yue-Feng ^{[3
,5
]}

Ge, Ri-Li ^{[1
,2
]}

Wang, Li-Sheng ^{[3
,5
,6
]}

机构：

[1] Qinghai Univ, Minist Chinese Educ, High Altitude Med, Xining 810001, Peoples R China

[2] Qinghai Univ, Res Ctr High Altitude Med, Xining 810001, Peoples R China

[3] Beijing Inst Radiat Med, Dept Expt Hematol, Beijing 100850, Peoples R China

[4] Qinghai Prov Peoples Hosp, Xining 810007, Peoples R China

[5] Beijing Inst Radiat Med, Beijing Key Lab Radiobiol, Beijing 100850, Peoples R China

[6] Jilin Univ, Sch Nursing, Changchun 130021, Jilin, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2017年 / 351卷 / 01期

基金：

中国国家自然科学基金;

关键词：

miR-486; Hypoxia; TF-1; cells; Exosomes; Erythroid differentiation; STEM-CELLS; MICRORNA-486-5P;

D O I：

10.1016/j.yexcr.2016.12.023

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

MicroRNAs (miRNAs) regulate the hypoxia-induced erythroid differentiation of hematopoietic cells. In this study, we identified that miR-486 was a rapid response miRNA to hypoxia in erythroleukemia TF-1 cells. Hypoxia exposure increased both intracellular and miR-486 levels of TF-1 cells. Ectopic miR-486 expression enhanced the growth and erythroid differentiation of TF-1 cells, whereas miR-486 inhibition suppressed their growth and erythroid differentiation. Treatment of TF-1 and cord blood CD34+ cells with exogenous containing miR-486 resulted in an increase of intracellular miR-486 level and enhanced erythroid differentiation. Furthermore, we identified that Sirt1 is a miR-486 target gene which modulates hypoxia-induced erythroid differentiation of TF-1 cells. Thus we identified a novel miRNA regulatory network that contributes to hypoxia-induced erythroid differentiation of hematopoietic cells.

引用

页码：74 / 81

页数：8

共 29 条

[1]

[Anonymous], PLOS ONE

[2]

Aging and disease: connections to sirtuins [J].