Minimally invasive dynamic imaging of ions and metabolites in living cells

被引：32

作者：

Fehr, M ^{[1
]}

Ehrhardt, DW ^{[1
]}

Lalonde, S ^{[1
]}

Frommer, WB ^{[1
]}

机构：

[1] Carnegie Inst Sci, Stanford, CA 94305 USA

来源：

CURRENT OPINION IN PLANT BIOLOGY | 2004年 / 7卷 / 03期

关键词：

D O I：

10.1016/j.pbi.2004.03.015

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

By 2010, it is expected that biochemical functions will be assigned to many of the products of the approximately 30 000 Arabidopsis genes. Moreover, systematic analysis of mutants will provide insight into the biological function of the gene products. Metabolomic technologies complement these approaches by testing for changes in cellular ion and metabolite patterns, providing essential information for the construction of cellular and whole-plant models of metabolism. However, one important set of information that is especially relevant for multicellular organisms is still lacking, that is, knowledge of the cellular and subcellular variation in metabolite levels. The recent development of protein-based nanosensors for metabolites will help to close this gap by providing a set of tools that can be used to determine cytosolic and subcellular metabolite levels in real time using fluorescence-based microscopy. A major challenge for the future is the application of these nanosensors to quantify metabolite levels in plant cells and tissues.

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收藏

页码：345 / 351

页数：7

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