Molecular cloning and characterization of cDNA encoding fibrinolytic enzyme-3 from earthworm Eisenia foetida

被引：17

作者：

Dong, GQ ^{[1
]}

Yuan, XL ^{[1
]}

Shan, YJ ^{[1
]}

Zhao, ZH ^{[1
]}

Chen, JP ^{[1
]}

Cong, YW ^{[1
]}

机构：

[1] Beijing Inst Radiat Med, Dept Expt Therapy ARS, Beijing 100850, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2004年 / 36卷 / 04期

关键词：

earthworm; Eisenia foetida; earthworm fibrinolytic enzyme; cloning; expression; refolding;

D O I：

10.1093/abbs/36.4.303

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The earthworm fibrinolytic enzyme-3 (EFE-3, GenBank accession No: AY438622), from the earthworm Eisenia foetida, is a component of earthworm fibrinolytic enzymes. In this study, cDNA encoding the EFE-3 was cloned by RT-PCR. The cDNA contained an open reading frame of 741 nucleotides, which encoded a deduced protein of 247 amino acid residues, including signal sequences. EFE-3 showed a high degree of homology to earthworm (Lumbricus rebullus) proteases F-III-1, F-III-2, and bovine trypsin. The recombinant EFE-3 was expressed in E. coli as inclusion bodies, and the gene encoding the native form of EFE-3 was expressed in COS-7 cells in the medium. Both the refolding product of inclusion bodies and the secreted protease could dissolve the artificial fibrin plate.

引用

页码：303 / 308

页数：6

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