Determination of ochratoxin a in cereals and feed by SAX-SPE clean up and LC fluorimetric detection

被引：11

作者：

Biancardi, A

Riberzani, A

机构：

[1] Department of Chemistry, Ist. Zooprofilattico Sperim. D.

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1996年 / 19卷 / 15期

关键词：

D O I：

10.1080/10826079608014025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a sensitive, reliable and highly specific method for the determination of ochratoxin A (OA) in cereals and animal feed. The samples were extracted in acidified acetonitrile and, they were then thoroughly purified by a new procedure combining a pH-controlled liquid-liquid partition with a strong anionic exchange solid-phase extraction. Both RP-TLC and RP-HPLC methods for semi-quantitative and quantitative determination have been described respectively, together with a RP-HPLC confirmation procedure, via conversion to OA-methyl ester. The TLC and HPLC determination limits were 8.0 ng/g and 0.10 ng/g respectively. The mean recovery from spiked samples was 95%.

引用

页码：2395 / 2407

页数：13

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