Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays

被引：103

作者：

Dai, HY ^{[1
]}

Meyer, M ^{[1
]}

Stepaniants, S ^{[1
]}

Ziman, M ^{[1
]}

Stoughton, R ^{[1
]}

机构：

[1] Rosetta Inpharmat, Kirkland, WA 98034 USA

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 16期

关键词：

D O I：

10.1093/nar/gnf085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hybridization kinetics were found to be significantly different for specific and non-specific binding of labeled cRNA to surface-bound oligonucleotides on microarrays. We show direct evidence that in a complex sample specific binding takes longer to reach hybridization equilibrium than the non- specific binding. We find that this property can be used to estimate and to correct for the hybridization contributed by non-specific binding. Useful applications are illustrated including the selection of superior oligonucleotides, and the reduction of false positives in exon identification.

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页数：8

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