Comprehensive transcriptomic and proteomic characterization of human mesenchymal stem cells reveals source specific cellular markers

被引：108

作者：

Billing, Anja M. ^{[1
]}

Ben Hamidane, Hisham ^{[1
]}

Dib, Shaima S. ^{[1
]}

Cotton, Richard J. ^{[1
]}

Bhagwat, Aditya M. ^{[1
]}

Kumar, Pankaj ^{[1
]}

Hayat, Shahina ^{[1
]}

Yousri, Noha A. ^{[1
]}

Goswami, Neha ^{[1
]}

Suhre, Karsten ^{[1
]}

Rafii, Arash ^{[1
]}

Graumann, Johannes ^{[1
]}

机构：

[1] Weill Cornell Med Qatar, Div Res, Doha, Qatar

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

ZINC-FINGER PROTEIN; BONE-MARROW; STROMAL CELLS; AMINO-ACIDS; CORD BLOOD; DIFFERENTIATION; EXPRESSION; THERAPY; TRANSPLANTATION; IDENTIFICATION;

D O I：

10.1038/srep21507

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Mesenchymal stem cells (MSC) are multipotent cells with great potential in therapy, reflected by more than 500 MSC-based clinical trials registered with the NIH. MSC are derived from multiple tissues but require invasive harvesting and imply donor-to-donor variability. Embryonic stem cell-derived MSC (ESC-MSC) may provide an alternative, but how similar they are to ex vivo MSC is unknown. Here we performed an in depth characterization of human ESC-MSC, comparing them to human bone marrow-derived MSC (BM-MSC) as well as human embryonic stem cells (hESC) by transcriptomics (RNA-seq) and quantitative proteomics (nanoLC-MS/MS using SILAC). Data integration highlighted and validated a central role of vesicle-mediated transport and exosomes in MSC biology and also demonstrated, through enrichment analysis, their versatility and broad application potential. Particular emphasis was placed on comparing profiles between ESC-MSC and BM-MSC and assessing their equivalency. Data presented here shows that differences between ESC-MSC and BM-MSC are similar in magnitude to those reported for MSC of different origin and the former may thus represent an alternative source for therapeutic applications. Finally, we report an unprecedented coverage of MSC CD markers, as well as membrane associated proteins which may benefit immunofluorescence-based applications and contribute to a refined molecular description of MSC.

引用

页数：15

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