Cold transiently activates calcium-permeable channels in Arabidopsis mesophyll cells

被引：85

作者：

Carpaneto, Armando

Ivashikina, Natalya

Levchenko, Victor

Krol, Elzbieta

Jeworutzki, Elena

Zhu, Jian-Kang

Hedrich, Rainer ^{[1
]}

机构：

[1] Univ Wurzburg, Julius Von Sachs Inst Biosci, Dept Mol Plant Physiol & Biophys, D-97082 Wurzburg, Germany

[2] CNR, Inst Biophys, I-16149 Genoa, Italy

[3] Univ Wales, Sch Biol Sci, Bangor LL57 2UW, Gwynedd, Wales

[4] Marie Curie Sklodowska Univ, Dept Biophys, Inst Biol, PL-20033 Lublin, Poland

[5] Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[6] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

来源：

PLANT PHYSIOLOGY | 2007年 / 143卷 / 01期

关键词：

D O I：

10.1104/pp.106.090928

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Living organisms are capable of discriminating thermal stimuli from noxious cold to noxious heat. For more than 30 years, it has been known that plant cells respond to cold with a large and transient depolarization. Recently, using transgenic Arabidopsis (Arabidopsis thaliana) expressing the calcium-sensitive protein aequorin, an increase in cytosolic calcium following cold treatment was observed. Applying the patch-clamp technique to Arabidopsis mesophyll protoplasts, we could identify a transient plasma membrane conductance induced by rapid cooling. This cold-induced transient conductance was characterized as an outward rectifying 33 pS nonselective cation channel. The permeability ratio between calcium and cesium was 0.7, pointing to a permeation pore.3.34 angstrom (phi of cesium). Our experiments thus provide direct evidence for the predicted but not yet measured cold-activated calcium-permeable channel in plants.

引用

页码：487 / 494

页数：8

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