Immune modulation by probiotic strains:: Quantification of phagocytosis of Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout (Oncorhynchus mykiss) using a radiolabelling assay

被引：48

作者：

Balcazar, Jose Luis ^{[1
]}

Vendrell, Daniel ^{[1
]}

de Blas, Ignacio ^{[1
]}

Ruiz-Zarzuela, Imanol ^{[1
]}

Girones, Olivia ^{[1
]}

Muzquiz, Jose Luis ^{[1
]}

机构：

[1] Univ Zaragoza, Lab Fish Pathol, E-50013 Zaragoza, Spain

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2006年 / 29卷 / 5-6期

关键词：

Aeromonas salmonicida; phagocytosis; leukocytes; rainbow trout; probiotics;

D O I：

10.1016/j.cimid.2006.09.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

A method is described for the rapid and sensitive assay of phagocytosis that utilizes radioactively labelled bacteria. With this method, we observed that phagocytosis of heat-inactivated Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout fed with different viable probiotics (Lactococcus lactis subsp. lactis, Lactobacillus sakei, and Leuconostoc mesenteroides) was significantly higher (P < 0.05) after 2 weeks of probiotic-feeding than the control group. However, only phagocytosis of live A. salmonicida by the leukocytes isolated from gut of rainbow trout fed with L. lactis subsp. lactis was significantly higher (P < 0.05) than the control group. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：335 / 343

页数：9

共 32 条

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