Preparation of human cDNAs encoding expanded polyglutamine repeats

被引:15
作者
Peters, MF
Ross, CA
机构
[1] Johns Hopkins Univ, Sch Med, Dept Psychiat, Mol Neurobiol Lab,Div Neurobiol, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
[3] Johns Hopkins Univ, Sch Med, Program Cellular & Mol Med, Baltimore, MD 21205 USA
关键词
polyglutamine; trinulceotide repeat; CAG repeat; Huntington's disease; type IIS restriction enzyme;
D O I
10.1016/S0304-3940(99)00758-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
At least eight neurodegenerative diseases result from expansions of polyglutamine tracts encoded by CAG trinucleotide repeats. Although polyglutamine diseases typically have onset after age 50 in humans, these diseases can be modeled in animals and in cell culture by using highly expanded repeats to accelerate the pathogenesis. Unfortunately, current methods for preparing recombinant constructs with large glutamine tracts either a Iter the coding region adjacent to the repeat or yield highly unstable pure CAG repeats. We have developed a technique for expanding repeats that results in a more stable mix of CAG and CAA glutamine codons. We expect th is technique to allow rapid preparation of highly expand repeats suitable for stable animal and cell culture models for any of the polyglutamine repeat diseases. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:129 / 132
页数:4
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