Astrocytes express type VIII collagen during the repair process of brain cold injury

被引：32

作者：

Hirano, S

Yonezawa, T

Hasegawa, H

Hattori, S

Greenhill, NS

Davis, PF

Sage, EH

Ninomiya, Y

机构：

[1] Okayama Univ, Grad Sch Med & Dent, Dept Mol Biol & Biochem, Okayama 7008558, Japan

[2] Nippi Res Inst Biomatrix, Adachi Ku, Tokyo 1208601, Japan

[3] Wellington Sch Med & Hlth Sci, Dept Med, Bioact Investigat Grp BIG, Wellington, New Zealand

[4] Hope Heart Inst, Dept Vasc Biol, Seattle, WA 98104 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 317卷 / 02期

基金：

日本学术振兴会;

关键词：

extracellular matrix; type VIII collagen; astrocyte; cold injury model; brain; transforming growth factor-B1; glial scar; glial fibrillary acidic protein; adhesion; migration;

D O I：

10.1016/j.bbrc.2004.03.049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recognized for the first time upregulation of type VIII collagen gene expression during the repair process in the mouse brain cold injury model. Immunohistochemical staining showed that type VIII collagen expression was around the necrotic region, where reactive astrocytes are frequently observed. Cultured astrocytes demonstrated a high expression of type VIII collagen genes. TGF-beta1 enhanced the expression of both alpha1(VIII) and alpha2(VIII) genes by astrocytes in culture. Further, we tested selected biological activities of type VIII collagen, compared with those of type I, IV, and V collagens and fibronectin. Astrocytes adhered to type VIII collagen via receptors requiring metal ions. Astrocyte migration on type VIII collagen was more stimulated than that observed on the other ECM molecules. These data indicate that type VIII collagen plays an important role in glial scar formation during the repair process by astrocytes. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：437 / 443

页数：7

共 41 条

[11] The α1(VIII) and α2(VIII) collagen chains form two distinct homotrimeric proteins in vivo [J].