Isoliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells

被引:115
作者
Jung, Jae In
Lim, Soon Sung
Choi, Hyun Ju
Cho, Han Jin
Shin, Hyun-Kyung
Kim, Eun Ji
Chung, Won-Yoon
Park, Kwang-Kyun
Park, Jung Han Yoon [1 ]
机构
[1] Hallym Univ, Dept Nutr & Food Sci, Chunchon 200702, South Korea
[2] Hallym Univ, Silver Biotechnol Res Ctr, Chunchon 200702, South Korea
[3] Yonsei Univ, Coll Dent, Dept Oral Biol, Seoul 120752, South Korea
关键词
Bcl-2; caspase; apoptosis; cytochrome C; prostate cancer cells;
D O I
10.1016/j.jnutbio.2005.11.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Isoliquiritigenin (ISL), a simple chalcone derivative, 4,2',4'-trihydroxychalcone, found in licorice, shallot and bean sprouts, has been reported to have chemoprotective effects. To examine the effects of ISL on the growth of prostate cancer cells, we cultured MAT-LyLu (MLL) rat and DU145 human prostate cancer cells with various concentrations (0-20 mu mol/L) of ISL. Treatment of the cells with increasing concentrations of ISL led to dose-dependent decreases in the viable cell numbers in both DU145 and MLL cells (P <.05). Hoechst 33258 dye staining of condensed nuclei and annexin V binding to surface phosphatidylserine revealed increased numbers of apoptotic cells after ISL treatment. Western blot analysis revealed that ISL increased the levels of membrane-bound Fas ligand (FasL), Fas, cleaved casapse-8, truncated Bid (tBid), Bax and Bad in DU145 cells (P <.05). Isoliquiritigenin increased the percentage of cells with depolarized mitochondrial membranes, in a concentration-dependent manner (P <.05). Isoliquiritigenin induced the release of cytochrome c and Smac/Diablo from the mitochondria into the cytoplasm (P <.05). Isoliquiritigenin dose-dependently increased the levels of cleaved caspsase-9, caspase-7, caspase-3 and poly(ADP-ribose) polymerase (P <.05). The present results indicate that ISL inhibits prostate cancer cell growth by the induction of apoptosis, which is mediated through mitochondrial events, which are associated with an evident disruption of the mitochondrial membrane potential, and the release of cytochrome c and Smac/Diablo, and the activation of caspase-9. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:689 / 696
页数:8
相关论文
共 43 条
  • [21] Isoliquiritigenin inhibits the growth of prostate cancer
    Kanazawa, M
    Satomi, Y
    Mizutani, Y
    Ukimura, O
    Kawauchi, A
    Sakai, T
    Baba, M
    Okuyama, T
    Nishino, H
    Miki, T
    [J]. EUROPEAN UROLOGY, 2003, 43 (05) : 580 - 586
  • [22] Update on hormone-refractory prostate cancer
    Kasamon, KM
    Dawson, NA
    [J]. CURRENT OPINION IN UROLOGY, 2004, 14 (03) : 185 - 193
  • [23] Programmed cell death: alive and well in the new millennium
    Kaufmann, SH
    Hengartner, MO
    [J]. TRENDS IN CELL BIOLOGY, 2001, 11 (12) : 526 - 534
  • [24] The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis
    Kluck, RM
    BossyWetzel, E
    Green, DR
    Newmeyer, DD
    [J]. SCIENCE, 1997, 275 (5303) : 1132 - 1136
  • [25] ANTIFOLDING ACTIVITY OF HSP60 COUPLES PROTEIN IMPORT INTO THE MITOCHONDRIAL MATRIX WITH EXPORT TO THE INTERMEMBRANE SPACE
    KOLL, H
    GUIARD, B
    RASSOW, J
    OSTERMANN, J
    HORWICH, AL
    NEUPERT, W
    HARTL, FU
    [J]. CELL, 1992, 68 (06) : 1163 - 1175
  • [26] Mitochondrial control of cell death
    Kroemer, G
    Reed, JC
    [J]. NATURE MEDICINE, 2000, 6 (05) : 513 - 519
  • [27] Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis
    Li, HL
    Zhu, H
    Xu, CJ
    Yuan, JY
    [J]. CELL, 1998, 94 (04) : 491 - 501
  • [28] Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
    Li, P
    Nijhawan, D
    Budihardjo, I
    Srinivasula, SM
    Ahmad, M
    Alnemri, ES
    Wang, XD
    [J]. CELL, 1997, 91 (04) : 479 - 489
  • [29] Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors
    Luo, X
    Budihardjo, I
    Zou, H
    Slaughter, C
    Wang, XD
    [J]. CELL, 1998, 94 (04) : 481 - 490
  • [30] Apoptosis induced by isoliquiritigenin in human gastric cancer MGC-803 cells
    Ma, J
    Fu, NY
    Pang, DB
    Wu, WY
    Xu, AL
    [J]. PLANTA MEDICA, 2001, 67 (08) : 754 - 757