Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia

被引:23
作者
Li, W
Jin, K [1 ]
Nagayama, T
He, X
Chang, J
Minami, M
Graham, SH
Simon, RP
Greenberg, DA
机构
[1] Buck Ctr Res Aging, Novato, CA 94948 USA
[2] Univ Pittsburgh, Sch Med, Dept Neurol, Pittsburgh, PA 15261 USA
关键词
apoptosis-linked gene 2; cerebral ischemia; apoptosis; programmed cell death; gene expression;
D O I
10.1016/S0306-4522(99)00531-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Calcium is an important mediator of programmed cell death induced by transient cerebral ischemia, and calcium-binding proteins have been implicated in calcium-regulated signal transduction. Apoptosis-linked gene 2 is a calcium-binding protein required for cell death induced by different apoptotic stimuli. By Western blot analysis, we found that apoptosis-linked gene 2 protein was expressed in normal brains, and that expression increased in ischemic brains after 20 or 90 min of transient focal cerebral ischemia. Immunocytochemistry showed increased apoptosis-linked gene 2 protein expression in frontal cortex, a region where neurons underwent ischemic stress but still survived, after 20 or 90 min of focal cerebral ischemia. Apoptosis-linked gene 2 protein was also up-regulated in the ischemic border-zone of parietal cortex 24 h after 20 min of focal ischemia, and was remarkably over-expressed in the caudate-putamen and parietal cortex, (where cells are destined to die) 24 h after 90 min of ischemia. The expression pattern of apoptosis-linked gene 2 protein was similar to that of deoxyribonucleic acid damage detected by Klenow labeling assay. Our results suggest that apoptosis-linked gene 2 may be involved in the regulation of cell death after transient focal cerebral ischemia. (C) 2000 IBRO. Published by Elsevier Science Ltd.
引用
收藏
页码:161 / 168
页数:8
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