DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific

被引:16
作者
Torres, L
López-Rodas, G
Latasa, MU
Carretero, MV
Boukaba, A
Rodríguez, JL
Franco, L
Mato, JM [1 ]
Avila, MA
机构
[1] Univ Navarra, Dept Med Interne, Div Hepatol & Terapia Gen, Pamplona 31008, Spain
[2] Univ Valencia, Dept Bioquim & Biol Mol, E-46100 Valencia, Spain
关键词
methionine adenosyltransferase; gene expression; DNA methylation; histone acetylation; anti-acetylated H4;
D O I
10.1016/S1357-2725(99)00140-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet). In mammals MAT activity derives From two separate genes which display a tissue-specific pattern of expression, While MAT1A is expressed only in the adult liver, MAT2A is expressed in non-hepatic tissues, The mechanisms behind the selective expression of these two, genes are not fully understood. In the present report we have evaluated MAT1A and MAT2A methylation in liver and in other tissues, such as kidney, by methylation-sensitive restriction enzyme digestion of genomic DNA, Our data indicate that,MAT1A is hypomethylated in liver and hypermethylated in non-expressing tissues. The opposite situation is found for;MAT2A. Additionally, histones associated to MAT1A and MAT2A genes showed enhanced let els of acetylation in expressing tissues (two-fold for MAT1A and 3.5-fold for MAT2A liver and kidney respectively). These observations support a role for chromatin structure and its modification in the tissue-specific expression of both MAT genes, (C) 2000 Elsevier Science Ltd, All rights reserved.
引用
收藏
页码:397 / 404
页数:8
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