Retention of ionizable compounds in high-performance liquid chromatography -: 14.: Acid-base pK values in acetonitrile-water mobile phases

被引：113

作者：

Espinosa, S ^{[1
]}

Bosch, E ^{[1
]}

Rosés, M ^{[1
]}

机构：

[1] Univ Barcelona, Dept Quim Analit, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 964卷 / 1-2期

关键词：

mobile phase compositions; retention behaviour; acidity; pK(a); dissociation constants; carboxylic acids; amines; phenols; pyridines;

D O I：

10.1016/S0021-9673(02)00558-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Linear relationships between (s)(s)pK(a) values in acetonitrile-water mixtures and (w)(w)pK(a), values in pure water have been established for five families of compounds: aliphatic carboxylic acids, aromatic carboxylic acids, phenols, amines, and pyridines. The parameters (slope and intercept) of the linear correlations have been related with acetonitrile-water composition, The proposed equations allow accurate estimation of the pK(a) values of any member of the studied families at any acetonitrile-water composition up to 60% of acetonitrile in volume (100% for pyridines). Conversely, the same equations can be used to estimate aqueous pK(a) values from chromatographic pK(a) values obtained from any acetonitrile-water mobile phase between the composition range studied. Estimation of pK(a) values have been tested with chromatographic literature data. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：55 / 66

页数：12

共 33 条

[1] A potentiometric study of acid-base equilibria of substituted pyridines in acetonitrile [J].

Augustin-Nowacka, D ;

Chmurzyñski, L .

ANALYTICA CHIMICA ACTA, 1999, 381 (2-3) :215-220

[2] ACID-BASE EQUILIBRIA AND ASSAY OF BENZODIAZEPINES IN ACETONITRILE MEDIUM [J].

BARBOSA, J ;

SANZNEBOT, V .

TALANTA, 1989, 36 (08) :837-842

[3] Standardization of potentiometric sensors and acid-base behaviour of a series of bases in nitrobenzene [J].

Barrón, D ;

Buti, S ;

Barbosa, J .

ANALYTICA CHIMICA ACTA, 2000, 403 (1-2) :349-354

[4]

BATES RG, 1973, DETERMINATION PH

[5] Retention of ionizable compounds on high-performance liquid chromatography -: III.: Variation of pK values of acids and pH values of buffers in acetonitrile-water mobile phases [J].

Bosch, E ;

Espinosa, S ;

Rosés, M .

JOURNAL OF CHROMATOGRAPHY A, 1998, 824 (02) :137-146

[6] IONIC EQUILIBRIA IN NEUTRAL AMPHIPROTIC SOLVENTS - RESOLUTION OF ACID STRENGTH IN TERT-BUTYL ALCOHOL [J].

BOSCH, E ;

ROSES, M .

TALANTA, 1989, 36 (06) :627-632

[7] Retention of ionizable compounds on HPLC. pH scale in methanol-water and the pK and pH values of buffers [J].

Bosch, E ;

Bou, P ;

Allemann, H ;

Roses, M .

ANALYTICAL CHEMISTRY, 1996, 68 (20) :3651-3657

[8] IONIC EQUILIBRIA IN NEUTRAL AMPHIPROTIC SOLVENTS - STRUCTURAL EFFECTS ON DISSOCIATION-CONSTANTS OF SEVERAL SUBSTITUTED PHENOLS AND MERCAPTOPYRIMIDINES IN ISOPROPYL-ALCOHOL [J].

BOSCH, E ;

RAFOLS, C ;

ROSES, M .

TALANTA, 1989, 36 (12) :1227-1231

[9] Retention of ionizable compounds on HPLC.: 4.: Mobile-phase pH measurement in methanol/water [J].

Canals, I ;

Portal, JA ;

Bosch, E ;

Rosés, M .

ANALYTICAL CHEMISTRY, 2000, 72 (08) :1802-1809

[10] Retention of ionizable compounds on HPLC.: Modelling retention in reversed-phase liquid chromatography as a function of pH and solvent composition with methanol-water mobile phases [J].

Canals, I ;

Portal, JA ;

Rosés, M ;

Bosch, E .

CHROMATOGRAPHIA, 2002, 55 (9-10) :565-571

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