Transepithelial transport of oligopeptides in the human intestinal cell, Caco-2

被引：143

作者：

Shimizu, M

Tsunogai, M

Arai, S

机构：

[1] Dept. of Appl. Biological Chemistry, Grad. Sch. of Agric. and Life Sci., University of Tokyo

[2] Dept. of Appl. Biological Chemistry, Grad. Sch. of Agric. and Life Sci., University of Tokyo, Tokyo 113, Bunkyo-ku

来源：

PEPTIDES | 1997年 / 18卷 / 05期

关键词：

bradykinin; Caco-2; intestinal epithelial cell; oligopeptide; transcytosis; paracellular transport;

D O I：

10.1016/S0196-9781(97)00002-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transepithelial transport of oligopeptides (of more than 4 residues) was studied by using human intestinal Caco-2 cell monolayers. The susceptibility to the brush-border peptidases was observed to be one of the primary factors which decide the transport rate. The apical-to-basolateral transport mechanism was investigated by using bradykinin and GGYR which were resistant to cellular peptidases. The intracellular pathway, probably the adsorptive transcytosis, was suggested to be involved in the transport of bradykinin and its analogues, the transport rate being particularly dependent on the hydrophobic properties of the peptides. On the other hand, the tetrapeptide such as GGYR was suggested to be transported mainly via the paracellular pathway. (C) 1997 Elsevier Science Inc.

引用

页码：681 / 687

页数：7

共 46 条

[1] QUANTITATIVE APPROACHES TO DELINEATE PARACELLULAR DIFFUSION IN CULTURED EPITHELIAL-CELL MONOLAYERS [J].