Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery

被引：65

作者：

Mikami, Y

Okano, H

Sakaguchi, M

Nakamura, M

Shimazaki, T

Okano, HJ

Kawakami, Y

Toyama, Y

Toda, M

机构：

[1] Keio Univ, Sch Med, Neuroimmunol Res Grp, Shinjuku Ku, Tokyo 1608582, Japan

[2] Keio Univ, Sch Med, Dept Orthopaed Surg, Tokyo 1608582, Japan

[3] Keio Univ, Sch Med, Dept Physiol, Tokyo 1608582, Japan

[4] Keio Univ, Sch Med, Dept Neurosurg, Tokyo 1608582, Japan

[5] Keio Univ, Sch Med, Inst Adv Med Res, Div Cellular Signaling, Tokyo 1608582, Japan

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 2004年 / 76卷 / 04期

关键词：

dendritic cells; spinal cord injury; neural stem cells; neurogenesis; regeneration;

D O I：

10.1002/jnr.20086

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We report a treatment for spinal cord injury involving implantation of dendritic cells (DCs), which act as antigen-presenting cells in the immune system. The novel mechanisms underlying this treatment produce functional recovery. Among the immune cells tested, DCs showed the strongest activity inducing proliferation and survival of neural stem/progenitor cells (NSPCs) in vitro. Furthermore, in DC-implanted adult mice, endogenous NSPCs in the injured spinal cord were activated for mitotic de novo neurogenesis. These DCs produced neurotrophin-3 and activated endogenous microglia in the injured spinal cord. Behavioral analysis revealed the locomotor functions of DC-implanted mice to have recovered significantly as compared to those of control mice. Our results suggest that DC-implantation exerts trophic effects, including activation of endogenous NSPCs, leading to repair of the injured adult spinal cord. (C) 2004 Wiley-Liss, Inc.

引用

页码：453 / 465

页数：13

共 72 条

[61] Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell [J].