Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs

被引：23

作者：

Alves, Endrigo G. L. ^{[1
,2
]}

Serakides, Rogeria ^{[2
]}

Boeloni, Jankerle N. ^{[2
]}

Rosado, Isabel R. ^{[2
]}

Ocarino, Natalia M. ^{[2
]}

Oliveira, Humberto P. ^{[2
]}

Goes, Alfredo M. ^{[3
]}

Rezende, Cleuza M. F. ^{[2
]}

机构：

[1] Univ Uberaba UNIUBE, Curso Med Vet, Uberaba, Brazil

[2] Univ Fed Minas Gerais, Escola Vet, NCT TCA Dept Clin & Cirurgia, Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Immunol, Belo Horizonte, MG, Brazil

来源：

BMC VETERINARY RESEARCH | 2014年 / 10卷

关键词：

Mesenchymal stem cells; Osteogenic differentiation; Dogs; PHYSICAL-ACTIVITY; STROMAL CELLS; DIFFERENTIATION; CANINE; RATS;

D O I：

10.1186/s12917-014-0190-y

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

090604 [动物药学];

摘要：

Background: The aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC). Results: MTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points. Conclusions: It was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.

引用

页数：9

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