New method to determine antioxidant activity of polyphenols

被引：92

作者：

Chaillou, Lucrecia L. ^{[1
]}

Nazareno, Monica A. ^{[1
]}

机构：

[1] Univ Nacl Santiago del Estero, Fac Agron & Agronind, Inst Ciencias Quim, RA-4200 Santiago Del Estero, Argentina

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 22期

关键词：

antioxidant activity; beta-carotene bleaching; lipoxygenase; polyphenols; flavonoids; phenolic acids; DPPH;

D O I：

10.1021/jf061729f

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The capacity of polyphenolic compounds to reduce the beta-carotene-linoleic acid cooxidation enzymatically induced by soybean lipoxygenase was assayed to determine their comprehensive antioxidant ability. The inhibition of the coupled oxidation is a well-known spectrophotometric method for the antioxidant activity measurement. A modification of this method is proposed to reduce assay time and to gain simplicity. The antioxidant abilities of several polyphenols were determined, and quercetin and sinapic acid were most active. These results were compared to those obtained by the DPPH center dot procedure to evaluate the free radical scavenging contribution to the total protective action. The highest values of antiradical activity were found for ellagic and quercetin.

引用

页码：8397 / 8402

页数：6

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