Functional genomics of the endocrine pancreas - The pancreas clone set and PancChip, new resources for diabetes research

被引：64

作者：

Scearce, LM

Brestelli, JE

McWeeney, SK

Lee, CS

Mazzarelli, J

Pinney, DF

Pizarro, A

Stoeckert, CJ

Clifton, SW

Permutt, MA

Brown, J

Melton, DA

Kaestner, KH

机构：

[1] Univ Penn, Dept Genet, Philadelphia, PA 19104 USA

[2] Univ Penn, Ctr Bioinformat, Philadelphia, PA 19104 USA

[3] Harvard Univ, Dept Mol & Cellular Biol, Boston, MA 02115 USA

[4] Washington Univ, Dept Internal Med, St Louis, MO USA

[5] Washington Univ, Genome Sequencing Ctr, St Louis, MO USA

来源：

DIABETES | 2002年 / 51卷 / 07期

关键词：

D O I：

10.2337/diabetes.51.7.1997

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Over the past 5 years, microarrays have greatly facilitated large-scale analysis of gene expression levels. Although these arrays were not specifically geared to represent tissues and pathways known to be affected by diabetes, they have been used in both type 1 and type 2 diabetes research. To prepare a tool that is particularly useful in the study of type 1 diabetes, we have assembled a nonredundant set of 3,400 clones representing genes expressed in the mouse pancreas or pathways known to be affected by diabetes. We have demonstrated the usefulness of this clone set by preparing a cDNA glass microarray, the PancChip, and using it to analyze pancreatic gene expression from embryonic day 14.5 through adulthood in mice. The clone set and corresponding array are useful resources for diabetes research.

引用

页码：1997 / 2004

页数：8

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