Compositional properties of human cDNA libraries: Practical implications

被引：8

作者：

Arhondakis, Stilianos ^{[1
]}

Clay, Oliver ^{[1
]}

Bernardi, Giorgio ^{[1
]}

机构：

[1] Staz Zool Anton Dohrn, Lab Mol Evolut, I-80121 Naples, Italy

来源：

FEBS LETTERS | 2006年 / 580卷 / 24期

关键词：

EST; GC expression level; GC biases; Sfi I;

D O I：

10.1016/j.febslet.2006.09.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The strikingly wide and bimodal gene distribution exhibited by the human genome has prompted us to study the correlations between EST-counts (expression levels) and base composition of genes, especially since existing data are contradictory. Here we investigate how cDNA library preparation affects the GC distributions of ESTs and/or genes found in the library, and address consequences for expression studies. We observe that strongly anomalous GC distributions often indicate experimental biases or deficits during their preparation. We propose the use of compositional distributions of raw ESTs from a cDNA library, and/or of the genes they represent, as a simple and effective tool for quality control. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5772 / 5778

页数：7

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