Targeted integration of a dominant neoR marker into a 2-to 3-Mb human minichromosome and transfer between cells

被引：8

作者：

Au, HC

Mascarello, JT

Scheffler, IE ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA

[2] Childrens Hosp, Dept Genet, San Diego, CA USA

来源：

CYTOGENETICS AND CELL GENETICS | 1999年 / 86卷 / 3-4期

关键词：

D O I：

10.1159/000015338

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The physical and genetic characterization of a stable human minichromosome in a Chinese hamster hybrid cell is described. The minichromosome is 2-3 Mb in size, is linear, and contains a complementing SDHC gene. It is derived from a human chromosome 1, including the centromere, some pericentric heterochromatin from 1q12, and 1-2 Mb of 1q21. Genomic DNA surrounding the SDHC gene was used to construct a targeting vector with a selectable drug resistance marker (neo(R)); the marker was then successfully integrated into the minichromosome. With the new selectable marker, the 8.2.3 minichromosome could be transferred into mouse LMTK- and 3T3 TK- cells.

引用

页码：194 / 203

页数：10