Capillary electrophoresis-fluorescence line-narrowing system for on-line structural characterization of molecular analytes

被引:27
作者
Jankowiak, R
Zamzow, D
Ding, W
Small, GJ
机构
[1] IOWA STATE UNIV SCI & TECHNOL,US DOE,AMES LAB,AMES,IA 50011
[2] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011
关键词
D O I
10.1021/ac960251j
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We have demonstrated, for the first time, that capillary electrophoresis (CE) can be interfaced with low-temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization. Detection by laser-induced fluorescence spectroscopy, under fluorescence non-line-narrowing and Line-narrowing conditions, provides three-dimensional electropherograms and FLN spectra, which lead to significantly improved overall resolution and allow for structural characterization (''fingerprinting'') of molecular analytes. This novel CE-FLN system consists of a modular CE system, instrumentation for FLN spectroscopy, and a specially designed capillary cryostat (CC). An absorbance detector serves to determine the migration rates of analytes. After the 77 K fluorescence-based electropherogram is generated, the temperature of the capillary is lowered to 4.2 K for high-resolution FLN characterization. Automated translation of the CG and capillary in the direction of the capillary axis allows the separated analytes to be sequentially characterized by fluorescence spectroscopy as the capillary is translated through the laser excitation region. Detection of fluorescence from stationary CE-separated analytes significantly improves the accuracy of quantitation and structural characterization. We believe that this interfacing represents an exciting addition to the rapidly evolving field of CE, providing a new and powerful tool for chemical analysis. The first application of the CE-FLN system to a mixture of polycyclic aromatic hydrocarbons is presented; prospects and future applications of CE-FLN are briefly addressed.
引用
收藏
页码:2549 / 2553
页数:5
相关论文
共 41 条
[1]   GLASS-FORMING COMPOSITION REGIONS AND GLASS TRANSITION TEMPERATURES FOR AQUEOUS ELECTROLYTE SOLUTIONS [J].
ANGELL, CA ;
SARE, EI .
JOURNAL OF CHEMICAL PHYSICS, 1970, 52 (03) :1058-&
[2]   Conformational studies of depurinating DNA adducts from syn-dibenzo[a,l]pyrene diolepoxide [J].
Ariese, F ;
Small, GJ ;
Jankowiak, R .
CARCINOGENESIS, 1996, 17 (04) :829-837
[3]   Cyclodextrin-modified capillary electrophoresis: Determination of polycyclic aromatic hydrocarbons in contaminated soils [J].
Brown, RS ;
Luong, JHT ;
Szolar, OHJ ;
Halasz, A ;
Hawari, J .
ANALYTICAL CHEMISTRY, 1996, 68 (02) :287-292
[4]   Single-molecule detection in capillary electrophoresis: Molecular shot noise as a fundamental limit to chemical analysis [J].
Chen, DY ;
Dovichi, NJ .
ANALYTICAL CHEMISTRY, 1996, 68 (04) :690-696
[5]   FLUORESCENCE LINE NARROWING SPECTROMETRY OF NUCLEOSIDE POLYCYCLIC AROMATIC HYDROCARBON ADDUCTS ON THIN-LAYER CHROMATOGRAPHIC PLATES [J].
COOPER, RS ;
JANKOWIAK, R ;
HAYES, JM ;
LU, PQ ;
SMALL, GJ .
ANALYTICAL CHEMISTRY, 1988, 60 (24) :2692-2694
[6]   Detection of attomolar concentrations of alkaline phosphatase by capillary electrophoresis using laser-induced fluorescence detection [J].
Craig, DB ;
Wong, JCY ;
Dovichi, NJ .
ANALYTICAL CHEMISTRY, 1996, 68 (04) :697-700
[7]   IDENTIFICATION AND QUANTITATION OF 7,12-DIMETHYLBENZ[A]ANTHRACENE-DNA ADDUCTS FORMED IN MOUSE SKIN [J].
DEVANESAN, PD ;
RAMAKRISHNA, NVS ;
PADMAVATHI, NS ;
HIGGINBOTHAM, S ;
ROGAN, EG ;
CAVALIERI, EL ;
MARSCH, GA ;
JANKOWIAK, R ;
SMALL, GJ .
CHEMICAL RESEARCH IN TOXICOLOGY, 1993, 6 (03) :364-371
[8]   DETERMINATION OF INTRACELLULAR SPECIES AT THE LEVEL OF A SINGLE ERYTHROCYTE VIA CAPILLARY ELECTROPHORESIS WITH DIRECT AND INDIRECT FLUORESCENCE DETECTION [J].
HOGAN, BL ;
YEUNG, ES .
ANALYTICAL CHEMISTRY, 1992, 64 (22) :2841-2845
[9]   SINGLE-CELL ANALYSIS AT THE LEVEL OF A SINGLE HUMAN ERYTHROCYTE [J].
HOGAN, BL ;
YEUNG, ES .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1993, 12 (01) :4-9
[10]   CAPILLARY ARRAY ELECTROPHORESIS USING LASER-EXCITED CONFOCAL FLUORESCENCE DETECTION [J].
HUANG, XHC ;
QUESADA, MA ;
MATHIES, RA .
ANALYTICAL CHEMISTRY, 1992, 64 (08) :967-972