Chemical tethering of motile bacteria to silicon surfaces

被引：18

作者：

Bearinger, Jane P. ^{[1
]}

Dugan, Lawrence C. ^{[1
]}

Wu, Ligang ^{[1
]}

Hill, Haley ^{[2
]}

Christian, Allen T. ^{[3
]}

Hubbell, Jeffrey A. ^{[4
,5
]}

机构：

[1] Lawrence Livermore Natl Lab, CMELS, Livermore, CA 94550 USA

[2] Northwestern Univ, Dept Chem, Evanston, IL USA

[3] Monsanto Co, St Louis, MO USA

[4] Ecole Polytech Fed Lausanne, Inst Bioengn, Lausanne, Switzerland

[5] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, Lausanne, Switzerland

来源：

BIOTECHNIQUES | 2009年 / 46卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

E; coli; tethering; patterning; microarrays; imaging; host-pathogen response; drug discovery; biofuel cells; MICROBIAL FUEL-CELLS; ELECTRICITY-GENERATION; CRYOELECTRON MICROSCOPY; PROTEIN ADSORPTION; BACILLUS-SUBTILIS; QUANTUM DOTS; BIOSENSORS; GROWTH; MICROORGANISMS; METABOLITES;

D O I：

10.2144/000113073

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.

引用

页码：209 / +

页数：7

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