Identification of a common gene signature for type H cytokine-associated myeloid cells elicited in vivo in different pathologic conditions

被引:131
作者
Ghassabeh, Gholamreza Hassanzadeh
De Baetselier, Patrick
Brys, Lea
Noel, Wim
Van Ginderachter, Jo A.
Meerschaut, Sofie
Beschin, Alain
Brombacher, Frank
Raes, Geert
机构
[1] Vrije Univ Brussel VIB, Cellular & Mol Immunol Lab, Dept Mol & Cellular Interact, B-1050 Brussels, Belgium
[2] Univ Cape Town, Fac Hlth Sci, ZA-7700 Rondebosch, South Africa
关键词
D O I
10.1182/blood-2005-04-1485
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Compared with type I cytokine-associated myeloid (M1) cells, the molecular repertoire and mechanisms underlying functional properties of type II cytokine-associated myeloid (M2) cells are poorly characterized. Moreover, most studies have been limited to in vitro-elicited M2 cells. Here, comparative gene expression profiling of M1 and M2 cells, elicited in murine models of parasitic infections and cancer, yielded a common signature for in vivo-induced M2 populations independent of disease model, mouse strain, and organ source of cells. Some of these genes, such as cadherin-1, selenoprotein P, platelet-activating factor acetylhydrolase, and prosaposin, had not been documented as associated with M2. Overall, the common signature genes provide a molecular basis for a number of documented or suggested properties of M2, including immunomodulation, downregulation of inflammation, protection against oxidative damage, high capacity for phagocytosis, and tissue repair. Interestingly, several common M2 signature genes encode membrane-associated markers that could be useful for the identification and isolation of M2. Some of these genes were not induced by IL-4/IL-13 or IL-10 under various in vitro settings and thus were missed in approaches based on in vitro-activated cells, validating our choice of in vivo models for expression profiling of myeloid cells.
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页码:575 / 583
页数:9
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