Increased apoptosis in U937 cells over-expressing lipocortin 1 (annexin I)

被引:30
作者
Canaider, S
Solito, E
de Coupade, C
Flower, RJ
Russo-Marie, F
Goulding, NJ
Perretti, M
机构
[1] WHRI, Dept Biochem Pharmacol, London EC1M 6BQ, England
[2] Inst Cochin Genet Mol, U332 INSERM, F-75014 Paris, France
关键词
lipocortin; 1; annexin I; apoptosis; U937; cells; transfection;
D O I
10.1016/S0024-3205(00)00500-2
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The potential involvement of endogenous lipocortin 1 in the process of cellular apoptosis, particularly in cells of the myelo-monocytic lineage, has been investigated. U937 cells were transfected either with an antisense or a sense DNA for Lipocortin 1 and the stable clones 36.4AS clone (20-40% lower Lipocortin 1 levels) and 15S (30% higher lipocortin 1 levels) were obtained. Cell apoptosis was induced by incubation with tumor necrosis factor-a: optimal responses were observed within a 24 h incubation period at a 5 ng/ml concentration. Apoptosis was assessed both morphologically, by annexin V binding and cell cycle analysis with propidium iodide. Whilst no consistent difference was seen between wild type cells and clone 36.4AS, a higher incidence of apoptosis (ranging from +30% to +60%) was observed in the 15S clone. Release of arachidonic acid from loaded cells was promoted by 24 h incubation with the cytokine, and a higher degree of release was measured in the 15S clone. These data indicate that endogenous intracellular lipocortin 1 is involved in the promotion of apoptosis in cells of the myelo-monocytic derivation. (C) 2000 Elsevier Science Inc.
引用
收藏
页码:PL265 / PL270
页数:6
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