Inhibition of pathogenic Salmonella enteritidis growth mediated by Escherichia coli microcin J25 producing strains

被引:48
作者
Portrait, V [1 ]
Gendron-Gaillard, S [1 ]
Cottenceau, G [1 ]
Pons, AM [1 ]
机构
[1] Univ La Rochelle, Lab Genie Prot & Cellulaire, F-17042 Nantes 01, France
关键词
microcin J25; Salmonella; mixed cultures;
D O I
10.1139/cjm-45-12-988
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
For the first time, microcin-producing strains showing inhibitory activities against enteropathogen Salmonella enteritidis were isolated from poultry intestinal contents. Among the numerous strains isolated, two strains of Escherichia coli, named J02 and J03, showing the greatest activities against S. enteritidis, were studied. Biochemical tests and purification identified the main antagonist compound produced as microcin J25. In order to evaluate the protective potential of E. coli J02 and J03 against S. enteritidis infection, the ability of these strains to inhibit growth of S. enteritidis was investigated in mixed culture. A strong antagonist activity was obtained with a preculture phase of the active strain in minimal medium before incubation with S. enteritidis. In a bioreactor experiment simulating the chicken gastric and intestinal tract environment, a mixture of the two strains E. coli J02 and J03, provided an enhanced inhibitory effect. Microcinogenic strain activities were not affected by bile, pancreatic enzymes addition, or acidic conditions. These results suggest the relevant role of microcin-producing microorganisms in microbial intestinal ecology. To conclude, this study shows that microcin J25 strains could exert a beneficial protective effect against S. enteritidis growth in situ.
引用
收藏
页码:988 / 994
页数:7
相关论文
共 25 条
[11]   Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin [J].
Drago, L ;
Gismondo, MR ;
Lombardi, A ;
deHaen, C ;
Gozzini, L .
FEMS MICROBIOLOGY LETTERS, 1997, 153 (02) :455-463
[12]   STRUCTURE AND MODE OF ACTION OF MICROCIN 7, AN ANTIBACTERIAL PEPTIDE PRODUCED BY ESCHERICHIA-COLI [J].
GARCIABUSTOS, JF ;
PEZZI, N ;
MENDEZ, E .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1985, 27 (05) :791-797
[13]   REGULATORY EFFECTS OF BIFIDOBACTERIA ON THE GROWTH OF OTHER COLONIC BACTERIA [J].
GIBSON, GR ;
WANG, X .
JOURNAL OF APPLIED BACTERIOLOGY, 1994, 77 (04) :412-420
[14]  
HERRERO M, 1986, J GEN MICROBIOL, V132, P393
[15]   Antimicrobial effects of amikacin therapy on experimentally induced Salmonella typhimurium infection in fowls [J].
Itoh, N ;
Kikuchi, N ;
Hiramune, T .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 1996, 58 (05) :425-429
[16]   ANTAGONISTIC EFFECTS OF LACTOBACILLI AND PEDIOCOCCI TO CONTROL INTESTINAL COLONIZATION BY HUMAN ENTEROPATHOGENS IN LIVE POULTRY [J].
JUVEN, BJ ;
MEINERSMANN, RJ ;
STERN, NJ .
JOURNAL OF APPLIED BACTERIOLOGY, 1991, 70 (02) :95-103
[17]   MICROCIN E492 FORMS ION CHANNELS IN PHOSPHOLIPID-BILAYER MEMBRANES [J].
LAGOS, R ;
WILKENS, M ;
VERGARA, C ;
CECCHI, X ;
MONASTERIO, O .
FEBS LETTERS, 1993, 321 (2-3) :145-148
[18]   Comparative study of the protective effect against Salmonella colonisation in newly hatched SPF chickens using live, attenuated Salmonella vaccine strains, wild-type Salmonella strains or a competitive exclusion product [J].
Methner, U ;
Barrow, PA ;
Martin, G ;
Meyer, H .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1997, 35 (03) :223-230
[19]  
MILLER JH, 1972, EXPT MOL GENETICS, P218
[20]  
Moreno F, 1995, Biotechnology, V28, P307