Genetic selection strategies for generating and characterizing catalysts

被引：12

作者：

Kast, P ^{[1
]}

Hilvert, D ^{[1
]}

机构：

[1] SCRIPPS RES INST, DEPT MOL BIOL, LA JOLLA, CA 92037 USA

来源：

PURE AND APPLIED CHEMISTRY | 1996年 / 68卷 / 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1351/pac199668112017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Molecular evolution is a powerful tool for exploring and creating catalytic function in biological macromolecules. This review focuses on examples and current strategies for the application of random mutagenesis of target genes coupled with direct selection in vivo. The technology is illustrated by the dissection of catalytic features in chorismate mutase. Future avenues for identifying and evolving catalytic antibodies are also discussed.

引用

页码：2017 / 2024

页数：8