Radiolabeling antibodies with holmium-166

被引：24

作者：

Dadachova, E

Mirzadeh, S

Smith, SV

Knapp, FFR

Hetherington, EL

机构：

[1] OAK RIDGE NATL LAB,NUCL MED GRP,OAK RIDGE,TN 37831

[2] AUSTRALIAN NUCL SCI & TECHNOL ORG,BIOMED & HLTH PROGRAM,MENAI,NSW 2234,AUSTRALIA

来源：

APPLIED RADIATION AND ISOTOPES | 1997年 / 48卷 / 04期

关键词：

D O I：

10.1016/S0969-8043(96)00269-2

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

We report the preliminary results from radiolabeling of a chelate-conjugated antibody with Ho-166 produced from the beta(-)-decay of Dy-166. Ho-166 was separated from mg quantities of Dy target by reverse phase ion-exchange chromatography employing a cation exchange HPLC column and 0.085 M alpha-HIBA at pH = 4.3 as eluent. Evaporation to dryness of Ho-166 fraction (up to 25 mL) and thermal decomposition of alpha-HIBA yielded Ho-166 in a dry state which was then solubilized in 0.5 mL of 0.1 M HCl. Subsequent radiolabeling of CHX-B-DTPA conjugated 135-14 monoclonal antibodies with purified Ho-166 was readily achieved with similar to 80% efficiency and with a specific activity of 3-4 mCi of Ho-166 per mg of protein. Ho-166-antibody conjugates are stable with regards to transferrin challenge for a period of 50 h. Further, it was shown that any Fe3+ ions present in alpha-HIBA as an impurity interfere with the labeling. (C) 1997 Elsevier Science Ltd.

引用

页码：477 / 481

页数：5

共 19 条

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