Direct identification of tryptophan in a mixture of amino acids by the naked eye

被引：18

作者：

Bao, Zhijuan ^{[1
]}

Sun, Shuna ^{[1
]}

Li, Jun ^{[1
]}

Chen, Xinqi ^{[1
]}

Dong, Saying ^{[1
]}

Ma, Huimin ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 40期

关键词：

amino acids; analytical methods; tautomerism; tryptophan; UV/Vis spectroscopy;

D O I：

10.1002/anie.200602144

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) Seeing is believing: Treatment of tryptophan with formic acid and hydrochloric acid generates a violet-blue color. The reaction is very specific, and can be used to identify tryptophan in a mixture of amino acids, or corroborate the existence of tryptophyl groups in peptides or proteins (see picture; A: blank; B: a mixture of 9 representative amino acids; C: tryptophan; D: mixture (B) and tryptophan; E: reduced glutathione; F: egg-white albumin). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6723 / 6725

页数：3

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