Inhibition of methyl linoleate autoxidation by phenolics and other related compounds under mild oxidative conditions

Methyl linoleate (MeLo) is a commercially available substrate widely used for studying the inhibitory properties of pure compounds and plant extracts against lipid oxidation. In this paper, 13 phenolic (benzoic and cinnamic acids, aldehydes and derivatives, and flavonoids) and other related compounds (butylated hydroxyanisole, butylated hydroxytoluene and tocopherols) as well as nine complex mixtures rich in phenolics (wines) or tocopherols (soybean oil extracts) were assayed for their inhibitory activity against MeLo autoxidation under mild conditions (40degreesC, darkness and atmospheric pressure). Samples were also assayed for their free radical-scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH.). Protocatechuic aldehyde, a compound that has not been previously evaluated by either of the two methods, showed the highest antioxidant activity. Some variations in the antioxidant ranking for some compounds were found between our results and those obtained by other authors using accelerated conditions for MeLo oxidation. Antioxidant activity of wines and soybean oil extracts was related to their richness in phenolics and tocopherols respectively. Correlation between antioxidant capacity measured by the MeLo and DPPH. methods was found for wines but not for the other samples studied. Therefore the measure of the free radical-scavenging capacity of a compound is not always a reliable indicator of its lipid oxidation inhibitory ability. (C) 2004 Society of Chemical Industry.