The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment of N-CoR

被引:73
作者
Masselink, H
Bernards, R
机构
[1] Netherlands Canc Inst, Div Mol Carcinogenesis, NL-1066 CX Amsterdam, Netherlands
[2] Netherlands Canc Inst, Ctr Biomed Genet, NL-1066 CX Amsterdam, Netherlands
关键词
BS69; BRAM1; N-CoR; E1A; MYND; repression;
D O I
10.1038/sj.onc.1203421
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
BS69 was first identified as a protein that interacts directly with the transactivation domain (conserved region 3) of the 289R adenovirus type 5 E1A protein. We show here that BS69 is a potent repressor of transcription, BS69 mediates repression, at least in part, through interaction with the co-repressor N-CoR, BS69 interacts with N-CoR through a MYND domain in its carboxyl terminus, A recently cloned splice variant of BS69, designated BRAM1, is also capable of interacting with N-CoR and E1A. but unlike BS69. is not able to repress transcription, indicating that N-CoR interaction is necessary but not sufficient for BS69 repression, Expression of E1A inhibits repression mediated by BS69, Our data suggest that BS69 participates in transcriptional repressor complexes and that E1A can modulate these complexes through interaction with BS69.
引用
收藏
页码:1538 / 1546
页数:9
相关论文
共 60 条
[41]   Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase [J].
Nagy, L ;
Kao, HY ;
Chakravarti, D ;
Lin, RJ ;
Hassig, CA ;
Ayer, DE ;
Schreiber, SL ;
Evans, RM .
CELL, 1997, 89 (03) :373-380
[42]   The transcriptional coactivators p300 and CBP are histone acetyltransferases [J].
Ogryzko, VV ;
Schiltz, RL ;
Russanova, V ;
Howard, BH ;
Nakatani, Y .
CELL, 1996, 87 (05) :953-959
[43]   Unique forms of human and mouse nuclear receptor corepressor SMRT [J].
Ordentlich, P ;
Downes, M ;
Xie, W ;
Genin, A ;
Spinner, NB ;
Evans, RM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :2639-2644
[44]   SMRTe, a silencing mediator for retinoid and thyroid hormone receptors-extended isoform that is more related to the nuclear receptor corepressor [J].
Park, EJ ;
Schroen, DJ ;
Yang, MZ ;
Li, H ;
Li, L ;
Chen, JD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3519-3524
[45]   HUMAN CYCLIN-A IS ADENOVIRUS E1A-ASSOCIATED PROTEIN-P60 AND BEHAVES DIFFERENTLY FROM CYCLIN-B [J].
PINES, J ;
HUNTER, T .
NATURE, 1990, 346 (6286) :760-763
[46]   MOLECULAR-CLONING AND CHARACTERIZATION OF A CELLULAR PHOSPHOPROTEIN THAT INTERACTS WITH A CONSERVED C-TERMINAL DOMAIN OF ADENOVIRUS E1A INVOLVED IN NEGATIVE MODULATION OF ONCOGENIC TRANSFORMATION [J].
SCHAEPER, U ;
BOYD, JM ;
VERMA, S ;
UHLMANN, E ;
SUBRAMANIAN, T ;
CHINADURAI, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (23) :10467-10471
[47]  
Schreiber-Agus N, 1998, BIOESSAYS, V20, P808, DOI 10.1002/(SICI)1521-1878(199810)20:10<808::AID-BIES6>3.0.CO
[48]  
2-U
[49]   AN AMINO-TERMINAL DOMAIN OF MXI1 MEDIATES ANTI-MYC ONCOGENIC ACTIVITY AND INTERACTS WITH A HOMOLOG OF THE YEAST TRANSCRIPTIONAL REPRESSOR SIN3 [J].
SCHREIBERAGUS, N ;
CHIN, L ;
CHEN, K ;
TORRES, R ;
RAO, G ;
GUIDA, P ;
SKOULTCHI, AI ;
DEPINHO, RA .
CELL, 1995, 80 (05) :777-786
[50]   Differential effects of nuclear receptor corepressor (N-CoR) expression levels on retinoic acid receptor-mediated repression support the existence of dynamically regulated corepressor complexes [J].
Soderstrom, M ;
Vo, A ;
Heinzel, T ;
Lavinsky, RM ;
Yang, WM ;
Seto, E ;
Peterson, DA ;
Rosenfeld, MG ;
Glass, CK .
MOLECULAR ENDOCRINOLOGY, 1997, 11 (06) :682-692