Molecular cloning and expression analysis of a novel member of the Disintegrin and Metalloprotease-Domain (ADAM) family

被引：13

作者：

Brachvogel, B

Reichenberg, D

Beyer, S

Jehn, B

von der Mark, K

Bielke, W

机构：

[1] Univ Erlangen Nurnberg, Dept Expt Med 1, D-91054 Erlangen, Germany

[2] Univ Erlangen Nurnberg, Dept Internal Med 3, Div Mol Immunol, D-91054 Erlangen, Germany

来源：

GENE | 2002年 / 288卷 / 1-2期

关键词：

Disintegrin-Metalloprotease; testase; development; testis; sequence; in situ; fertilization; sperm;

D O I：

10.1016/S0378-1119(02)00508-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We cloned and characterized the cDNA and the expression pattern of a novel member of the murine 'Disintegrin and Metalloprotease-Domain Family' (ADAM). The predicted protein sequence reveals highest homology to the testase-subgroup, composed of ADAM 24, ADAM 25 and ADAM 26 and is therefore called testase 4. Reverse transcription-polymerase chain reaction showed a strong expression of testase 4 in the adult testis, but not in any other organ or embryonic stage tested. Careful characterization by in situ hybridization confirmed specific expression of testase 4 in maturing sperm cells of 6-week-old mice, whereas no specific hybridization pattern was detectable in testes of 2.5-week-old mice. These data indicate a correlation between testase 4 expression and spermatogenesis and/or fertilization. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 210

页数：8

共 43 条

[21] Nielsen H, 1997, Int J Neural Syst, V8, P581, DOI 10.1142/S0129065797000537
[22] Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis
Pan, DJ
Rubin, GM
[J]. CELL, 1997, 90 (02) : 271 - 280
[23] An essential role for ectodomain shedding in mammalian development
Peschon, JJ
Slack, JL
Reddy, P
Stocking, KL
Sunnarborg, SW
Lee, DC
Russell, WE
Castner, BJ
Johnson, RS
Fitzner, JN
Boyce, RW
Nelson, N
Kozlosky, CJ
Wolfson, MF
Rauch, CT
Cerretti, DP
Paxton, RJ
March, CJ
Black, RA
[J]. SCIENCE, 1998, 282 (5392) : 1281 - 1284
[24] The ADAM gene family - surface proteins with adhesion and protease activity
Primakoff, P
Myles, DG
[J]. TRENDS IN GENETICS, 2000, 16 (02) : 83 - 87
[25] IDENTIFICATION AND PURIFICATION OF A SPERM SURFACE PROTEIN WITH A POTENTIAL ROLE IN SPERM EGG MEMBRANE-FUSION
PRIMAKOFF, P
HYATT, H
TREDICKKLINE, J
[J]. JOURNAL OF CELL BIOLOGY, 1987, 104 (01) : 141 - 149
[26] Processing of the Notch ligand delta by the metalloprotease kuzbanian
Qi, HL
Rand, MD
Wu, XH
Sestan, N
Wang, WY
Rakic, P
Xu, T
Artavanis-Tsakonas, S
[J]. SCIENCE, 1999, 283 (5398) : 91 - 94
[27] RAWLINGS ND, 1995, METHOD ENZYMOL, V248, P183
[28] KUZ, a conserved metalloprotease-disintegrin protein with two roles in Drosophila neurogenesis
Rooke, J
Pan, D
Xu, T
Rubin, GM
[J]. SCIENCE, 1996, 273 (5279) : 1227 - 1231
[29] Russell LD., 1990, HISTOLOGICAL HISTOPA
[30] THE NEIGHBOR-JOINING METHOD - A NEW METHOD FOR RECONSTRUCTING PHYLOGENETIC TREES
SAITOU, N
NEI, M
[J]. MOLECULAR BIOLOGY AND EVOLUTION, 1987, 4 (04) : 406 - 425

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