Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus

被引:70
作者
Chan, C. M. [1 ,2 ,3 ]
Tse, Herman [1 ,2 ,3 ]
Wong, S. S. Y. [1 ,2 ,3 ]
Woo, P. C. Y. [1 ,2 ,3 ]
Lau, S. K. P. [1 ,2 ,3 ]
Chen, L. [1 ,2 ,3 ]
Zheng, B. J. [1 ,2 ,3 ]
Huang, J. D. [1 ,2 ,3 ]
Yuen, K. Y. [1 ,2 ,3 ]
机构
[1] Dept Microbiol, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Beijing 100864, Peoples R China
关键词
Human coronavirus HKU1; Spike; Serorepidemiology; Neutralization antibody; SARS-COV; PROTECTIVE IMMUNITY; VACCINIA VIRUS; HONG-KONG; CHILDREN; ANTIBODY; PNEUMONIA; POLYPEPTIDE; SEQUENCE; RECEPTOR;
D O I
10.1016/j.jcv.2009.02.011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Human coronavirus HKU1 (HCoV-HKU1) is a recently identified coronavirus with a global distribution and known to cause mainly respiratory infections. Objectives: To investigate the seroepidemiology of HKU1 infections in our local population. Study design: An ELISA-based IgG antibody detection assay using recombinant HCoV-HKU1 nucleocapsid and spike (S) proteins (genotype A) were developed for the diagnosis of CoV-HKU1 infections, Additionally, a neutralization antibody assay using the HCoV-HKU1 pseudotyped virus was developed to detect the presence of neutralizing antibodies in serum with antibody positivity in an S protein-based ELISA. Results: Results of the recombinant S protein-based ELISA were validated with Western blot, immunofluorescence analysis and flow cytometry. The coupled results demonstrated good correlation with Spearmen's coefficient of 0.94. Seroepidemiological study in a local hospital-based setting using this newly developed ELISA showed steadily increasing HCoV-HKU1 seroprevalence in childhood and early adulthood, front 0% in the age group of <10 years old to a plateau of 21.6% in the age group of 31-40 years old. Conclusions: Our study demonstrated the usefulness of the S-based ELISA which could be applied to future epidemiological studies of HCoV-HKU1 in other localities. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:54 / 60
页数:7
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