Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells

被引：275

作者：

Tsang, Wing Pui ^{[1
]}

Kwok, Tim Tak ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Ctr Sci, Shatin, Hong Kong, Peoples R China

来源：

JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 2010年 / 21卷 / 02期

关键词：

EGCG; Tea polyphenols; miRNA; Bcl-2; Apoptosis; MICRORNA EXPRESSION; RNAS; POLYPHENOLS; CARCINOMA; TARGETS;

D O I：

10.1016/j.jnutbio.2008.12.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epigallocatechin gallate (EGCG) is a major type of green tea polyphenols and is known to have cancer prevention effect. MicroRNAs (miRNAs) are 19 to 25 nucleotides and are believed to be important in gene regulation. In the present study, the influence of EGCG on the expressions of miRNAs in human cancer cells was investigated as this has not yet been reported. By miRNA microarray analysis, EGCG treatment was found to modify the expressions of some of the miRNAs in human hepatocellular carcinoma HepG2 cells, 13 were up-regulated and 48 were down-regulated. miR-16 is one of the miRNAs up-regulated by EGCG and one of its target genes is confirmed to be the anti-apoptotic protein Bcl-2. EGCG treatment induced apoptosis and down-regulated Bcl-2 in HepG2 cells. Transfection with anti-miR-16 inhibitor suppressed miR-16 expression and counteracted the EGCG effects on Bcl-2 down-regulation and also induction of apoptosis in cells. Results from the present study confirm the role of miR-16 in mediating the apoptotic effect of EGCG and also support the importance of miRNAs in the regulation of the biological activity of EGCG. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：140 / 146

页数：7

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