Production of human antibody repertoires in transgenic mice

被引：44

作者：

Bruggemann, M ^{[1
]}

Taussig, MJ ^{[1
]}

机构：

[1] TRANSLOCUS THERAPEUT LTD, BABRAHAM INST, CAMBRIDGE CB2 4AT, ENGLAND

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0958-1669(97)80068-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic mice have been created that carry human immunoglobulin heavy and light chain genes in germline configuration and that have the corresponding endogenous genes silenced. The transgenes are either minigene constructs or large, almost authentic, transloci on yeast artificial chromosomes and undergo B-cell-specific DNA rearrangement and hypermutation in the mouse lymphoid tissue. Monoclonal antibodies with good affinities for human antigens have been obtained after immunisation. These mice may be a future source of human antibodies for therapy.

引用

页码：455 / 458

页数：4

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