Biomarker candidate identification in Yersinia pestis using organism-wide semiquantitative proteomics

被引：35

作者：

Hixson, Kim K.

Adkins, Joshua N.

Baker, Scott E.

Moore, Ronald J.

Chromy, Brett A.

Smith, Richard D.

McCutchen-Maloney, Sandra L.

Lipton, Mary S. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

[2] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

[3] Pacific NW Natl Lab, Chem & Biol Proc Dev Grp, Environm Technol Directorate, Richland, WA 99352 USA

[4] Lawrence Livermore Natl Lab, Biodef Div, Livermore, CA USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 11期

关键词：

biomarker; Yersinia; HPLC; mass spectrometry; relative quantitation;

D O I：

10.1021/pr060179y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The accurate mass and time tag mass spectrometry method and clustering analysis were used to compare the abundance change of 992 Yersinia pestis proteins under four contrasting growth conditions (26 and 37 degrees C, with or without Ca2+) that mimicked growth states in either a flea vector or mammalian host. Eighty-nine proteins were observed to have similar abundance change profiles to 29 known virulence associated proteins, providing identification of additional biomarker candidates. Eighty-seven hypothetical proteins, which clustered into 5 distinct clusters of like-protein abundance change, were identified as unique biomarkers related specifically to growth condition.

引用

页码：3008 / 3017

页数：10

共 48 条

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