Identification of mutations that disrupt phosphorylation-dependent nuclear export of cyclin D1

被引:107
作者
Benzeno, S.
Lu, F.
Guo, M.
Barbash, O.
Zhang, F.
Herman, J. G.
Klein, P. S.
Rustgi, A.
Diehl, J. A.
机构
[1] Univ Penn, Leonard & Madlyn Abramson Family Canc Res Inst, Dept Canc Biol, Philadelphia, PA 19104 USA
[2] Univ Penn, Ctr Canc, Philadelphia, PA 19104 USA
[3] Johns Hopkins Univ, Sch Med, Johns Hopkins Oncol Ctr, Dept Hematol Oncol, Baltimore, MD 21205 USA
[4] Univ Penn, Dept Med, Philadelphia, PA 19104 USA
关键词
cyclin D1; GSK-3; beta; CDK4; nuclear export; cancer;
D O I
10.1038/sj.onc.1209644
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although cyclin D1 is overexpressed in a significant number of human cancers, overexpression alone is insufficient to promote tumorigenesis. In vitro studies have revealed that inhibition of cyclin D1 nuclear export unmasks its neoplastic potential. Cyclin D1 nuclear export depends upon phosphorylation of a C-terminal residue, threonine 286, (Thr-286) which in turn promotes association with the nuclear exportin, CRM1. Mutation of Thr-286 to a non-phosphorylatable residue results in a constitutively nuclear cyclin D1 protein with significantly increased oncogenic potential. To determine whether cyclin D1 is subject to mutations that inhibit its nuclear export in human cancer, we have sequenced exon 5 of cyclin D1 in primary esophageal carcinoma samples and in cell lines derived from esophageal cancer. Our work reveals that cyclin D1 is subject to mutations in primary human cancer. The mutations identified specifically disrupt phosphorylation of cyclin D1 at Thr-286, thereby enforcing nuclear accumulation of cyclin D1. Through characterization of these mutants, we also de. ne an acidic residue within the C-terminus of cyclin D1 that is necessary for recognition and phosphorylation of cyclin D1 by glycogen synthase kinase-3 beta. Finally, through construction of compound mutants, we demonstrate that cell transformation by the cancer-derived cyclin D1 alleles correlates with their ability to associate with and activate CDK4. Our data reveal that cyclin D1 is subject to mutations in primary human cancer that specifically disrupt phosphorylation-dependent nuclear export of cyclin D1 and suggest that such mutations contribute to the genesis and progression of neoplastic growth.
引用
收藏
页码:6291 / 6303
页数:13
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