Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose

被引：50

作者：

Miyachi, Yusuke ^{[1
]}

Shimizu, Nobuaki ^{[2
]}

Ogino, Chiaki ^{[1
]}

Fukuda, Hideki ^{[1
]}

Kondo, Akihiko ^{[1
]}

机构：

[1] Kobe Univ, Dept Sci & Chem Engn, Grad Sch Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kanazawa Univ, Div Mat Sci, Grad Sch Neutral Sci & Technol, Kanazawa, Ishikawa 9201192, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2009年 / 19卷 / 13期

关键词：

DNA aptamer; SELEX; 8-OHdG; IN-VITRO SELECTION; RNA;

D O I：

10.1016/j.bmcl.2009.04.130

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). However, 8-OHdG was difficult to immobilize on a carrier for SELEX. Therefore, a DNA aptamer binding to 8-OHdG was selected using GMP-agarose as an analogue from a library of about 4(60) random ssDNA sources. As a result, three aptamer candidates were selected. Among the selected DNA aptamers, the No. 22 DNA aptamer exhibited a high affinity for 8-OHdG. The dissociation constant, K-D, of No. 22 DNA aptamer was on the order of 0.1 mu mol/L. This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：3619 / 3622

页数：4

共 16 条

[1] HAIRPIN AND PARALLEL QUARTET STRUCTURES FOR TELOMERIC SEQUENCES [J].

BALAGURUMOORTHY, P ;

BRAHMACHARI, SK ;

MOHANTY, D ;

BANSAL, M ;

SASISEKHARAN, V .

NUCLEIC ACIDS RESEARCH, 1992, 20 (15) :4061-4067

[2] SPECIFIC INTERACTION BETWEEN THE SELF-SPLICING RNA OF TETRAHYMENA AND ITS GUANOSINE SUBSTRATE - IMPLICATIONS FOR BIOLOGICAL CATALYSIS BY RNA [J].

BASS, BL ;

CECH, TR .

NATURE, 1984, 308 (5962) :820-826

[3] Isolation of high-affinity GTP aptamers from partially structured RNA libraries [J].

Davis, JH ;

Szostak, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (18) :11616-11621

[4] Methodology for urinary 8-hydroxy-2′-deoxyguanosine analysis by HPLC with electrochemical detection [J].

de Martinis, BS ;

Bianchi, MDLP .

PHARMACOLOGICAL RESEARCH, 2002, 46 (02) :129-131

[5] Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis [J].

Ekuni, Daisuke ;

Tomofuji, Takaaki ;

Tamaki, Naofumi ;

Sanbe, Toshihiro ;

Azuma, Tetsuji ;

Yamanaka, Reiko ;

Yamamoto, Tatsuo ;

Watanabe, Tatsuo .

ARCHIVES OF ORAL BIOLOGY, 2008, 53 (04) :324-329

[6] The thrombin binding aptamer GGTTGGTGTGGTTGG forms a bimolecular guanine tetraplex [J].

Fialová, M ;

Kypr, J ;

Vorlicková, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 344 (01) :50-54

[7] Measured and calculated CD spectra of G-quartets stacked with the same or opposite polarities [J].

Gray, Donald M. ;

Wen, Jin-Der ;

Gray, Carla W. ;

Repges, Rudolf ;

Repges, Charlotte ;

Raabe, Gerhard ;

Fleischhauer, Joerg .

CHIRALITY, 2008, 20 (3-4) :431-440

[8] Assays for cytokines using aptamers [J].

Guthrie, JW ;

Hamula, CLA ;

Zhang, HQ ;

Le, XC .

METHODS, 2006, 38 (04) :324-330

[9] A DNA APTAMER THAT BINDS ADENOSINE AND ATP [J].

HUIZENGA, DE ;

SZOSTAK, JW .

BIOCHEMISTRY, 1995, 34 (02) :656-665

[10] Aptameric enzyme subunit for homogeneous DNA sensing [J].

Ikebukuro, Kazunori ;

Yoshida, Wataru ;

Sode, Koji .

BIOTECHNOLOGY LETTERS, 2008, 30 (02) :243-252

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