Total Oxidant Status and Bone Resorption Biomarkers in Serum and Gingival Crevicular Fluid of Patients With Periodontitis

被引:76
作者
Baltacioglu, Esra [1 ]
Kehribar, Malike Aslan [1 ]
Yuva, Pinar [1 ]
Alver, Ahmet [2 ]
Atagun, Ozlem Sarac [1 ]
Karabulut, Erdem [3 ]
Akalin, Ferda Alev [4 ]
机构
[1] Karadeniz Tech Univ, Fac Dent, Dept Periodontol, TR-61080 Trabzon, Turkey
[2] Karadeniz Tech Univ, Fac Med, Dept Biochem, TR-61080 Trabzon, Turkey
[3] Hacettepe Univ, Fac Med, Dept Biostat, TR-06100 Ankara, Turkey
[4] Hacettepe Univ, Dept Periodontol, Fac Dent, Ankara, Turkey
关键词
Aggressive periodontitis; molecular biology; oxidative stress; periodontal disease; KAPPA-B LIGAND; TOTAL ANTIOXIDANT CAPACITY; LIPID-PEROXIDATION LEVELS; OXIDATIVE STRESS; RECEPTOR ACTIVATOR; REACTIVE OXYGEN; OSTEOCLAST DIFFERENTIATION; AGGRESSIVE PERIODONTITIS; OSTEOPROTEGERIN LEVELS; SUPEROXIDE-DISMUTASE;
D O I
10.1902/jop.2013.130012
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Background: In this study, the relationships between total oxidant status (TOS) and receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) levels and RANKL/OPG ratios in serum and gingival crevicular fluid (GCF) are investigated in patients with chronic (CP) and generalized aggressive (GAgP) periodontitis. Methods: Thirty patients with CP, 30 patients with GAgP, and 28 periodontally healthy controls were included in the study. After clinical measurements and samplings, serum and GCF TOS, RANKL, and OPG levels were determined by a novel automatic colorimetric method and enzyme-linked immunosorbent assays. Results: Serum and GCF TOS, RANKL, and RANKL/OPG values were higher in the periodontitis groups compared with controls, and they were also higher in the GAgP group than the CP group (except serum and GCF RANKL). Furthermore, serum and GCF OPG concentrations were lower in the periodontitis groups than in controls. Strong positive and negative correlations were observed between the periodontal parameters TOS and bone resorption biomarkers. Conclusions: The present results reveal that TOS, RANKL, and RANKL/OPG values are systemically and locally increased in periodontitis and that this increase is more evident in AgP than CP. These findings further suggest that oxidative stress is closely associated with the severity of periodontitis and bone resorption biomarkers.
引用
收藏
页码:317 / 326
页数:10
相关论文
共 52 条
[21]
FoxOs, Wnts and oxidative stress-induced bone loss: new players in the periodontitis arena? [J].
Galli, C. ;
Passeri, G. ;
Macaluso, G. M. .
JOURNAL OF PERIODONTAL RESEARCH, 2011, 46 (04) :397-406
[22]
Cytokines That Promote Periodontal Tissue Destruction [J].
Graves, Dana .
JOURNAL OF PERIODONTOLOGY, 2008, 79 (08) :1585-1591
[23]
Gumusx P, J PERIODONTOL, DOI [10.1902/jop.2013.120595, DOI 10.1902/J0P.2013.120595]
[24]
Osteoprotegerin, RANKL and bone turnover in postmenopausal osteoporosis [J].
Jabbar, Suhair ;
Drury, John ;
Fordham, John N. ;
Datta, Harish K. ;
Francis, Roger M. ;
Tuck, Stephen P. .
JOURNAL OF CLINICAL PATHOLOGY, 2011, 64 (04) :354-357
[25]
Resolution of inflammation in periodontitis [J].
Kantarci, A ;
Van Dyke, TE .
JOURNAL OF PERIODONTOLOGY, 2005, 76 (11) :2168-2174
[26]
Antioxidant α-lipoic acid inhibits osteoclast differentiation by reducing nuclear factor-κB DNA binding and prevents in vivo bone resorption induced by receptor activator of nuclear factor-κB ligand and tumor necrosis factor-α [J].
Kim, Hyon Jong ;
Chang, Eun-Ju ;
Kim, Hyun-Man ;
Lee, Seung Bok ;
Kim, Hyun-Duck ;
Kim, Ghi Su ;
Kim, Hong-Hee .
FREE RADICAL BIOLOGY AND MEDICINE, 2006, 40 (09) :1483-1493
[27]
Total antioxidant status and 8-hydroxy-2′-deoxyguanosine levels in gingival and peripheral blood of periodontitis patients [J].
Konopka, Tomasz ;
Krol, Katarzyna ;
Kopec, Waclaw ;
Gerber, Hanna .
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS, 2007, 55 (06) :417-422
[28]
Markers of bone destruction and formation and periodontitis in type 1 diabetes mellitus [J].
Lappin, David F. ;
Eapen, Bob ;
Robertson, Douglas ;
Young, Jenny ;
Hodge, Penny J. .
JOURNAL OF CLINICAL PERIODONTOLOGY, 2009, 36 (08) :634-641
[29]
A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation [J].
Lee, NK ;
Choi, YG ;
Baik, JY ;
Han, SY ;
Jeong, DW ;
Bae, YS ;
Kim, N ;
Lee, SY .
BLOOD, 2005, 106 (03) :852-859
[30]
LOE HARALD, 1963, ACTA ODONTOL SCAND, V21, P533, DOI 10.3109/00016356309011240